Effects of Ringer’s solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk

doi:10.12047/j.cjap.5750.2019.050

Abstract

Abstract: Objective: To quantitatively investigate the effects of Ringer’s solution with different concentrations of alcohol (1%～80%) on biphasic compound action potentials (AP) from frog sciatic nerve trunk, and their recoveries from alcohol effects. Methods: Individual segments of frog sciatic nerve trunk with a length of 6 to 8 cm were prepared. Ringer’s solution with different concentrations of alcohol (0%, 1%, 2%, 4%, 8%, 16%, 32%, 48%, 64% and 80%) was applied onto the segment of the trunk between the stimulus and ground electrodes via an agent reservoir which was newly armed in a nerve trunk shielded chamber for 5 minutes. The nerve trunk was respectively electro-stimulated to generate the biphasic compound AP which was recorded using the experimental system of BL-420F. This was followed by 5 times washout plus 5 min administration with Ringer’s solution before recovery recording of AP. Results: Compared to normal Ringer’s solution, Ringer’s solution with alcohol at ≤4% did not have dramatic impacts on the AP amplitude and conduction velocity, while Ringer’s solution with alcohol at ≥8% there was significant decrease in these two parameters. Ringer’s solution with alcohol at the conentrations of 16%, 32% and ≥48% could prevent a small proportion (30%), a large proportion (90%) and all (100%) of sciatic nerve trunks, respectively, from generating AP. Washout with normal Ringer’s solution after alcohol application at the concentration of ≤32%, AP could totally recover to normal status. While alcohol at the concentration of 48%, 64% and 80%, the probabilities to regenerate APs were 90%, 40% and 0%, and the AP amplitudes were decreased to 60%, 36% and 0%, respectively. After washout, AP conduction velocity showed no difference with alcohol at the concentration of ≤8% when compared with that before washout, while it could not be recovered to normal under alcohol at ≥16%. Conclusion: Ringer’s solution with different concentrations of alcohol exerts different effects on biphasic compound AP amplitude and conduction velocity. Hopefully, our findings could be helpful for the alcoholic usage and its recovery from alcoholic damage.

Key words: Ringer’s solution with different concentration of alcohol, bullfrog, sciatic nerve trunk, biphasic compound action potentials

HUANG Zhi-hua, WEI Pei-jian, JIANG Ling, CHEN Sui, CHENG Bi-hong, LIN Ying, WU Lin-geng, XU Qiu-xiong, WU Shao-wei, WANG Hai-yan, SHEN Jian-xin. Effects of Ringer’s solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk[J]. CJAP, 2019, 35(3): 232-238.

References

[1] Verbaten MN. Chronic effects of low to moderate alcohol consumption on structural and functional properties of the brain: beneficial or not[J]？ Hum Psychopharmacol, 2009, 24(3): 199-205.
[2] Mellion M., Gilchrist JM, de la Monte S. Alcohol-related peripheral neuropathy: nutritional, toxic, or both[J]？ Muscle Nerve, 2011, 43(3): 309-316.
[3] Ridley NJ, Draper B, Withall A. Alcohol-related dementia: an update of the evidence[J]. Alzheimers Res Ther, 2013, 5(1): 3-5.
[4] 崔海伦, 任汝静, 王刚. 酒精诱发的神经认知障碍:从对疾病认识的发展到诊疗的新进展[J]. 内科理论与实践, 2017, 12(2): 152-156.
[5] Yao LH, Yu HM, Xiong QP, et al. Cordycepin decreases compound action potential conduction of frog sciatic nerve in vitro involving Ca (2+) -dependent mechanisms[J]. Neural Plast, 2015, 2015: 927817.
[6] 胡还忠, 牟阳灵. 医学机能学实验教程(第4版)[M]. 4版. 北京: 科学出版社, 2016: 13, 36-37, 101-103.
[7] Hirao R, Fujita T, Sakai A, et al. Compound action potential inhibition produced by various antidepressants in the frog sciatic nerve[J]. Eur J Pharmacol, 2018, 819: 122-128.
[8] 张卫东, 郎庆玲. 乙醇对蟾蜍坐骨神经绝对不应期的影响[J]. 黑龙江医药科学, 2004, 27(3): 8-9.
[9] 邱梦君, 王仪丽, 万梦玲, 等. 浅谈三种高浓度非电解质溶液对蛙坐骨神经干动作电位的影响[J]. 当代医药论丛, 2015, 13(6): 229-230.
[10]仇银霞, 王洪磊. 乙醇对蟾蜍坐骨神经运动功能的影响[J]. 黑龙江畜牧兽医, 2015(20): 167-168.
[11]李小芳, 杨志松, 黄小富, 等. 乙醇与甲醇对中华大蟾蜍坐骨神经动作电位影响的比较研究[J]. 西华师范大学学报(自然科学版), 2009, 30(4): 392-397.
[12]翟心慧, 吴清华, 王志华, 等. 酒精对蟾蜍离体坐骨神经干动作电位的影响[J]. 平原大学学报, 2005, 22(6): 122-124.
[13]李润花. 不同因素对蟾蜍坐骨神经干动作电位的影响[J]. 太原师范学院学报(自然科学版), 2010, 9(4): 143-146.
[14]Kawasaki H, Mizuta K, Fujita T, et al. Inhibition by menthol and its related chemicals of compound action potentials in frog sciatic nerves[J]. Life Sci, 2013, 92(6-7): 359-367.
[15]Buyukakilli B, Comelekoglu U, Tataroglu C, et al. Reversible conduction block in isolated frog sciatic nerve by high concentration of bupivacaine[J]. Pharmacol Res, 2003, 47(3): 235-241.
[16]杜民, 周伟肖, 李涛, 等. 乙醇对牛蛙坐骨神经干动作电位影响的初步研究[J]. 吉林农业, 2013(3): 48.
[17]王波. 青风藤水煎液对蟾蜍坐骨神经干电生理的影响[J]. 中国应用生理学杂志, 2017, 33(4): 377-379.
[18]李海涛, 王俊亚, 张冬梅, 等. BL420F系统下神经干动作电位的引导[J]. 现代医药卫生, 2011, 27(12): 1920-1921.
[19]翟心慧, 王志华, 吴清华, 等. 咖啡碱对蟾蜍坐骨神经干动作电位和离体心脏活动的影响[J].中国应用生理学杂志, 2008, 24(4): 397-398.
[20]Li Z, Yang J, Liu J, et al. Reversible conduction block in isolated toad sciatic nerve by emulsified isoflurane[J]. Anesth Analg, 2010, 110(4): 1024-1029.