Intervention effect of Youguiwan on rats with knee osteoarthritis and its mechanism

doi:10.12047/j.cjap.5988.2020.109

CJAP ›› 2020, Vol. 36 ›› Issue (5): 511-516.doi: 10.12047/j.cjap.5988.2020.109

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Intervention effect of Youguiwan on rats with knee osteoarthritis and its mechanism

YAN Chun-lu^1,7, AN Fang-yu^1,6△, WANG Yong-feng³, JIN Hua⁴, ZHAO Chong-bo⁵, WANG Tong-xiang⁵, DENG Jie⁵, ZHANG Lei-lei⁸

1. Institute of Chinese and Western Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou 730000;2. Teaching Experiment Training Center;3. School of Basic Medical Science;4. Clinical College of Traditional Chinese Medicine;5. School of Clinical Medicine;
6. Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou 730000;
7. Key Laboratory of Dunhuang Medicine, Ministry of Education, Lanzhou 730000;
8. Centers for Disease Control and Prevention of Lanzhou, Lanzhou 730000, China

Received:2019-12-16 Revised:2020-06-18 Published:2021-02-25

Abstract

Abstract: Objective: To study the effects of Youguiwan on the osteoglycin (OGN), osteonectin (ON), fibrinogen 2 (FBN2) of articular cartilage tissue in the model of knee osteoarthritis (KOA). Methods: Sixty SD rats were randomly divided into six groups: sham control group, model group, glucosamine sulfate group, Youguiwan (high-dose, middle-dose and low-dose )group. The modified Hulth method was used to establish KOA models for 6 weeks. The sham control group and the model group were treated with normal saline. The rats in Youguiwan high-dose, middle-dose, low-dose groups were treated with Youguiwan at the doses of 4.8, 2.4, 1.2 g/kg by gavage respectively, and the glucosamine sulfate group was treated with glucosamine sulfate 0.17 g/kg. The rats were administrated for 8 weeks according to the dose. After intervening, articular cartilage of rats were obtained, the pathological changes were observed by using HE staining method, and Mankin score was evaluated. The expressions of OGN, ON and FBN2 in articular cartilage were detected by immunohistochemistry. The expression of GSK-3β in articular cartilage was detected by Western blot. Results: Compared with the sham control group, the Mankin score was obviously increased in the model group, the protein expression of FBN2 was increased significantly, yet the protein expressions of OGN, ON and GSK-3β were decreased significantly (P＜0.01), articular cartilage was seriously damaged, and chondrocytes were arranged in disorder. Compared with the model group, the Mankin score was declined obviously in the high-dose Youguiwan group, the protein expression of FBN2 was significantly decreased, but the protein expression of GSK-3β was significantly increased, the protein expressions of OGN and ON were significantly increased in the middle-dose and high-dose Youguiwan group (P＜0.05 or P＜0.01), cartilage structure was tended to be normal, the chondrocytes distribution was uneven, and articular cartilage surface was not smooth. Conclusion: Youguiwan can significantly improve the articular cartilage degeneration of KOA rats, its mechanism maybe raise OGN and ON protein expression level to promote the ossification and reconstruction of articular cartilage.

Key words: knee osteoarthritis, youguiwan, osteoglycin, osteonectin, fibrinogen 2

CLC Number:

R684.3

YAN Chun-lu, AN Fang-yu, WANG Yong-feng, JIN Hua, ZHAO Chong-bo, WANG Tong-xiang, DENG Jie, ZHANG Lei-lei. Intervention effect of Youguiwan on rats with knee osteoarthritis and its mechanism[J]. CJAP, 2020, 36(5): 511-516.

References

[1] Dowsey MM, Choong PF. The utility of outcome measures in total knee replacement surgery[J]. Int J Rheumatol, 2013(2013): 506-518.
[2] Price CC, Tanner JJ, Schmalfuss I, et al. A Pliot study evaluating presurgery neuroanan tomical biomarjers for postoperative cognitive Decline after total knee arthroplasty in older adults[J]. Anesthesiology, 2014, 120(3): 601-613.
[3] 于娟, 李莎莎, 叶莹仪, 等. 补肾活血方对兔膝骨性关节炎的治疗作用研究[J]. 中国实验方剂学杂志, 2014, 20(6): 171-174.
[4] 颜春鲁, 李盛华, 安方玉, 等. 右归丸对膝骨性关节炎模型鼠基质金属蛋白酶及炎性因子表达的影响[J]. 中国骨质疏松杂志, 2018, 24(5): 645-650.
[5] 王云峰, 白人骁, 张扬, 等. 改良Hulth 模型复制膝不同时期骨关节炎的实验研究[J]. 天津医科大学学报, 2009, 15(3): 400-404.
[6] Rogart JN, Barract HJ, Chichester CO. Articular collagen degrada -tion in the Hulth-Telhag model of osteoarthritis[J]. Osteoarthritis Cartilage, 1999, 7(6): 539-547.
[7] VandS JA, Geesink RGT, Van Dlaj, et al. The reliability of the Mankin score for osteoarthritis[J]. J Orthop Res, 1992, 10(1): 58-61.
[8] 潘建科, 杨伟毅, 刘军, 等. 龙鳖胶囊对膝骨关节炎大鼠软骨和滑膜病理的影响[J]. 中国中西医结合杂志, 2017, 37(7): 807-812.
[9] 邵洁琦, 曾志江, 杨敏, 等. 右归丸联合隔姜灸治疗阳虚寒凝型膝骨性关节炎的临床效果[J]. 中国医药导报, 2018, 15(10): 106-109.
[10]吴嵩, 廖佑荣, 吴和平, 等. 安络痛胶囊联合右归丸治疗阳虚寒凝型膝骨性关节炎疗效观察[J]. 药物流行病学杂志, 2016, 25(6): 342-345.
[11]Ameye L, Young MF. Mice deficient in small leucinerich proteoglycans: novel in vivo models for osteoporosis, osteoarthritis, Ehlers-Danlos syndrome, muscular dystrophy, and corneal diseases[J]. Glycobiology, 2002, 12(9): 107-116.
[12]Tasheva ES, Koester A, Paulsen AQ, et al. Mimecan/osteoglycin-deficient mice have collagen fibril abnormalities[J]. Mol Vis, 2002, 8: 407-415.
[13]Plaas AH, West LA, Thonar EJ, et al. Altered fine structures of corneal and skeletal keratan sulfate and chondroitin/dermatan sulfate in macular corneal dystrophy[J]. J Biol Chem, 2001, 276: 39788-39796.
[14]Tasheva ES, Maki CG, Conrad AH, et al. Transcriptional activation of bovine mimecan by p53 through an intronic DNA-binding site[J]. Biochem Biophys Acta, 2001, 1517: 333-338.
[15]叶青青, 王彦, 闫超. Osteoglycin对小鼠骨髓间充质干细胞诱导分化成骨细胞的作用和相关机制的研究[J]. 临床医药文献杂志, 2018, 96(5): 159- 161.
[16]De Ceuninck F, Marcheteau E, Berger S, et al. Assessment of some tools for the characteriza-tion of the human osteoarthritic cartilage proteome[J]. J Biomol Tech, 2005, 16(3): 256-265.
[17]Juchtmans N, Dhollander AA, Coudenys J, et al. Distinct dysregulation of the small leucine-rich repeat protein family in osteoarthritic acetabular labrum compared to articular cartilage[J]. Arthritis Rheumatol, 2015, 67(2): 435-441.
[18]Oshima Y, Coutts RD, Badlani NM, et al. Effect of light-emitting diode (LED) therapy on the development of-osteoarthritis-(OA) in a rabbit mode[J]. Biomed Pharmacother, 2011, 65(3): 224-229.
[19]汤译博, 赵亮, 苏佳灿. 骨粘连蛋白的理论研究与临床应用[J]. 中国组织工程研究与临床康复, 2010, 14 (28): 5264-5267.
[20]Wu J, Huang JF, Qin XX, et al. Platelet-rich plasma inhibits Wnt/β-catenin signaling in rabbit cartilage cells activated by IL-1β[J]. Int Immunopharmacol, 2018, 55: 282-289.
[21]Zheng W, Lin P, Ma Y, et al. Psoralen promotes the expression of cyclin D1 in chondrocytes via theWnt/β-catenin signaling pathway[J]. Int J Mol Med, 2017, 40(5): 1377-1384.
[22]Litherland GJ, Hui W, Elias MS, et al. Glycogen synthase kinase 3 inhibition stimulates human cartilage destruction and exacerbates murine osteoarthritis[J]. Arthritis Rheumatol, 2014, 66(8): 2175-2187.
[23]侯文斌, 韦嵩. 南星骨痛凝胶贴膏对兔膝骨关节炎关节形态及血清IL-1、TNF-α、MMP-13、TIMP-1表达的影响[J]. 辽宁中医杂志, 2020, 47(2): 184-187.
[24]孟祥东, 冯宇, 李福龙, 等. 盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制及其作用机理[J]. 中国中医骨伤科杂志, 2020, 28(2): 1-4.

Intervention effect of Youguiwan on rats with knee osteoarthritis and its mechanism

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