Abstract:Objective To investigate the distribution and drug sensitivity rates of microorganisms in mountain soil, so as to provide a basis for the prevention, diagnosis and treatment of infectious disease at Markam district, Yunnan province.Methods We selected sunny slope and shady slope of 4 mountains as sampling points at Markam district. The soil sampling was collected as zigzag. After culture, bacteria and fungi were isolated and identified by TREK DLAGNOSTIC SYSTEMS LTD automated microbial identification system. Susceptibility test was conducted by K-B method. Identification and susceptibility test of anaerobes was conducted by VITEK 2 System.Results The total amount of microorganism in shady slope 〔(7.34±0.59)×106 cfu/g〕 was significantly higher than that in sunny slope 〔(5.71±0.553)×106 cfu/g〕 (P<0.05). Only 2.5% of soil microorganism was fungi, while 97.5% was mainly bacteria (Gram-negative bacteria 56.5%, Gram-positive bacteria 36.7%, and anaerobes 4.3%). And most microorganisms were sensitive to commonly used antibiotics.Conclusion The amount of mountain soil microorganisms in shady slope is higher than in sunny slope at Markam district. The most microorganisms seperated are sensitive to commonly used antibiotics.
子建文, 李 倩, 唐 辉, 资云海, 张胜梅, 李晓霞. 云南芒康地区山脉阳坡面和阴坡面土壤微生物分布及药敏情况[J]. 解放军预防医学杂志, 2016, 34(1): 16-18.
ZI Jianwen, LI Qian, TANG Hui, ZI Yunhai, ZHANG Shengmei, LI Xiaoxia. Distribution and Drug Sensitivity of Mountain Soil Microorganismsat Markam District in Yunnan Province. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(1): 16-18.