牙龈卟啉单胞菌对感染流感病毒肺上皮细胞一氧化氮合酶的影响

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摘要目的观察牙龈卟啉单胞菌(Porphyromonas gingivalis，P.gingivalis)对感染流感病毒(H1N1)肺上皮细胞一氧化氮合酶的影响，以期为探讨牙周病在肺疾病发病机制中的作用提供临床依据。方法利用不同MOI值P.gingivalis与MOI值为2的H1N1共同感染于肺上皮细胞，未感染的细胞作为阴性对照。利用硝酸还原酶法测定上清液中NO的浓度;Westernblot检测诱导型一氧化氮合酶(iNOS)表达水平。结果各组细胞在4 h、8 h、12 h、24 h内NO生成均有增加。4 h、8 h、12 h、24 h后E组NO的量分别为41.404± 3.079、78.945±3.001、592.983±1.690、2360.660±4.199 μmol/L，8 h、12 h、24 h与对照组比较差异有显著性(P<0.05)。12 h、24 h P.gingivalis促进H1N1感染的肺上皮细胞iNOS蛋白水平的表达，与对照组相比差异有显著性(P<0.05)。结论 P.gingivalis与H1N1共同作用于肺上皮细胞能够促进iNOS的表达，最终促进NO的生成。

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关键词 ：牙龈卟啉单胞菌, 流感病毒, 肺上皮细胞, 一氧化氮, 一氧化氮合酶

Abstract：Objective To observe the impact of Porphyromonas gingivalis on the expression of nitric oxide synthase in pulmonary epithelial cells infected with influenza virus .Methods Pulmonary epithelial cells were infected with 2 MOIs (multiplicity of infection) of H1N1 or different MOIs of Porphyromonas gingivalis before being divided into groups A-E. Nitric oxide (NO) production was assessed by measuring the accumulation of nitrite in culture supernatants. The level of inducible nitricoxide synthase (iNOS) was detected by Western blot .Results NO production increased in each group at 4h, 8h, 12h, 24h. The level of NO production in group E at 4h, 8h, 12h, 24h was 41.404 3.079, 78.945 8h, 1, 592.9838h, 12, 2360.660h, and 12h umol/L, respectively, and NO production at 8h, 12h, 24h was significantly higher than that in control group (P<0.05). The expression of iNOS in pulmonary epithelial cells infected with Influenza virus was more significantly promoted by Porphyromonas gingivalis at 12h, 24h, and than in control group (P<0.05).Conclusion Porphyromonas gingivalis may increase the expression of iNOS, thus promoting the synthesis of NO.

Key words： porphyromonas gingivalis influenza virus pulmonary epithelial cells nitric oxide nitric oxide synthase

出版日期: 2016-09-14

基金资助:辽宁省科学计划项目(No.2013022029)

作者简介: 王圣男(1988-)，女，硕士研究生。从事牙周病与肺部疾病之间的关系研究。

引用本文:

王圣男，李新，李永刚，潘亚萍. 牙龈卟啉单胞菌对感染流感病毒肺上皮细胞一氧化氮合酶的影响[J]. 解放军预防医学杂志, 2016, 34(4): 489-492.
WANG Shengnan, LI Xin, LI Yonggang, PAN Yaping. Impact of Porphyromonas Gingivalis on Nitric Oxide Synthase Expression in Pulmonary Epithelial Cells Infected with Influenza Virus. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(4): 489-492.

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http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I4/489

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