7种有机磷农药对不同来源乙酰胆碱酯酶活性的抑制作用比较

全文: PDF(3035 KB)
输出: BibTeX | EndNote (RIS)

摘要目的研究7种不同有机磷农药对不同来源的乙酰胆碱酯酶的敏感性,筛选出适于指示这些农药的敏感酶。方法测定倍硫磷、草甘磷、对硫磷、马拉硫磷、毒死蜱、久效磷、甲拌磷7种有机磷农药对家蝇(Musca domestica)AChE、电鳗(Electrophorus electricus)电器官AChE活性。结果倍硫磷、草甘磷、对硫磷、毒死蜱、久效磷对家蝇AchE敏感,活性抑制率高于电鳗电器官AchE,在农药质量浓度为1000 μg/L时,抑制率最高,分别为85.14%、75.28%、40.94%、85.43%、55.68%;马拉硫磷、甲拌磷对电鳗电器官AchE敏感,抑制率高于家蝇AchE,在农药质量浓度为1000 μg/L时,抑制率最高,分别为45.29%、80.58%。结论倍硫磷、草甘磷、对硫磷、毒死蜱、久效磷对家蝇AChE敏感,马拉硫磷、甲拌磷对电鳗电器官AChE敏感。

	服务

	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ：有机磷农药, 乙酰胆碱酯酶活性, 抑制作用, 家蝇和电鳗

Abstract：Objective To study the sensitvity of seven types of organophosphorus pesticides to acetylcholinesterase(AChE) from different sources.Methods We detected the acetylcholinesterase (AChE) activity of electric organs of numbfish and Musca domestica to seven types of organophosphorus pesticides (fethion, glyphosate, parathion, chlorpyrifos, moncrotophos, malathion, phorate) .Results The result showed that the AChE activity of Musca domestica to fethion, glyphosate, parathion, chlorpyrifos and moncrotophos was higher than that of numbfish.The highest inhibition rates were 85.14%、75.28%、40.94%、and 85.43% respectively at 1000μg/L concentration.However, the AChE activity of numbfish to malathion and phorate was higher than that of Musca domestica, and the highest inhibition rates were 45.29%、80.58% respectively at the concentration of 1000μg/L.Conclusion The AChE of Musca domestica is much more sensitive to fethion, glyphosate, parathion, chlorpyrifos and moncrotophos while the AChE of electric organs of numbfish is much more sensitive to malathion and phorate.

Key words： organophosphorus pesticides acetylcholinesterase(AChE) activity inhibition numbfish and Musca domestica

收稿日期: 2015-04-24 出版日期: 2017-01-05

基金资助:乌鲁木齐市科技局应用开发研究计划(No.Y141310011)

通讯作者: thxxlz@sina.com

引用本文:

田华, 贾继民, 张建江, 常红, 邱尔臣, 楚敏. 7种有机磷农药对不同来源乙酰胆碱酯酶活性的抑制作用比较[J]. 解放军预防医学杂志, 2016, 34(6): 846-848.
TIAN Hua, JIA Jimin, ZHANG Jianjiang, CHANG Hong, QIU Erchen, CHU Min. Comparative Analysis of inhibition of Seven Orgnaophosphorus Pesticides to Activity of Acetylcholinesterase(AChE) from Different Sources. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(6): 846-848.

链接本文:

http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I6/846

[1]	林玉锁,龚瑞忠,朱忠林.农药与生态环境保护[M].北京:化学工业出版社,1999:52.
[2]	李元光,吴太虎,罗金辉,等.有机磷农药现场快速检测仪的研制[J].医疗卫生装备,2001,22(2):19.
[3]	WEISS C M.The deteminnation of cholinesterase in xhe brain tissile of three species of fresh water fish and its inactivation in vivo[J].Ecology,1958,39:194.
[4]	卢锋,乔玲.有机磷农药环境雌激素样的作用[J].解放军预防医学杂志,2004,22(5):338.
[5]	江藤守著,杨石先译.有机磷农药的有机化学和生物化学[M].北京:化学工业出版社,1981.
[6]	罗小红,李文强,李学惠,等.血液碱化对急性有机磷农药中毒的作用观察[J].解放军医药杂志,2013,25(9):82.
[7]	王小瑜.蔬菜中有机磷农药残留快速检测方法的研究[D].山东理工大学,2008.
[8]	ELLMAN G L.A new and rapid colorimetric determination of acetylcholinesterease activity[J].Biochem.Pharmacol,1961,7:88.
[9]	BRMIJN J D,HERMENSM J.Inhibition of acetylcholinesterase and acute toxicity of organophosphorous compounds to fish:a preliminary structure-activity analysis[J].Aquatic Toxicology,1993,24:257.
[10]	郑允.生化法检验杀虫剂残毒之简介[M].台湾农业试验所,1985.
[11]	GB/T 5009.145-2003.蔬菜中有机磷和氮基甲酸酯类农药残留量快速检测方法[S].2003.
[12]	刘向龙,于明克,左玉芝,等.血必净注射液联合磷酸肌酸钠对重度急性有机磷农药中毒患者心肌保护作用的临床观察[J].解放军医药杂志,2013,25(3):62.
[13]	余孝颖.有机磷农药对不同生物来源的胆碱酯酶选择性抑制的研究[J].环境科学,1996,17(4):41.
[14]	段世华.有机磷对胆碱酯酶作用影响的生化分析[J].吉安师专学报,1998,19(5):20.
[15]	徐家驹.有机磷杀虫剂的生化[J].生命的化学,1989,9:22.
[16]	宋国林,王宁华.乙酰胆碱酯酶法检测果蔬农药残留的研究[J].医疗卫生装备,2005,26(4):31.