绿原酸诱导肺癌细胞凋亡及其机制研究

摘要
参考文献
相关文章
Metrics

全文: PDF(3699 KB)
输出: BibTeX | EndNote (RIS)

摘要目的研究绿原酸对人肺癌A549细胞凋亡的作用及其机制,为临床应用提供依据。方法四氮唑盐(MTT)法检测不同浓度的绿原酸对A549细胞增殖的影响;流式细胞术检测细胞凋亡率;采用间接荧光标记法测定细胞内活性氧水平的变化;分光光度法检测绿原酸对表皮生长因子受体酪氨酸激酶(epidermal growth factor receptor tyrosine kinase,EGFR-TPK)、基质金属蛋白酶(matrix metalloproteinase 2,MMP-2)和氨肽酶N(aminopeptidase, APN)活性的变化。结果 MTT法检测显示,绿原酸在24 h、48 h和72 h时均可抑制A549细胞增殖,且呈浓度和时间依赖性关系趋势;绿原酸作用48 h后,细胞凋亡率呈现明显增高的趋势,与对照组比较差异有显著性(P<0.01);在100 μg/ml质量浓度的绿原酸作用下使细胞内活性氧(ROS)百分率达到了(42.32±3.3)%,与对照组相比比较差异有显著性(P<0.01);绿原酸对APN、EGFR-TPK和MMP-2半数抑制浓度(IC₅₀)分别为(8.12±0.78)、(29.43±2.76)和(1.54±0.13) μg/ml,显示出了较好的抑制效果。结论绿原酸可以抑制A549的增殖和转移,促进细胞凋亡,其凋亡机制可能与细胞内活性氧组分的增多及APN、EGFR-TPK和MMP-2活性降低有关。

	服务

	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ：绿原酸, 人肺癌A549, 凋亡, 活性氧组分, 表皮生长因子受体酪氨酸激酶, 基质金属蛋白酶, 氨肽酶N

Abstract：Objective To study the effect of chlorogenic acid on apoptosis of lung cancer A549 cells and its mechanism.Methods MTT assay was used to detect the cell proliferation induced by chlorogenic acid. Using the indirect fluorescent marker method, the reactive oxygen species (ROS) in cells was monitored by flow cytometry. The inhibitory effect of?chlorogenic acid on EGFR-TPK, APN, and MMP-2 was detected using spectrophotometric method.Results Chlorogenic acid inhibited the cell proliferation in a time- and concentration-dependent manner. After treatment with chlorogenic acid, the cell apoptosis rate and ROS activation rate were significantly increased (P<0.01). The half inhibition rate IC50 of chlorogenic acid on APN, EGFR-TPK and MMP-2 was (8.12 ± 0.78)、(29.43 ± 2.76) and (1.54 ± 0.13) μg/ml respectively.Conclusion Chlorogenic acid can inhibit A549 cell proliferation and induce apoptosis, which may be associated with the generation of ROS and decreased activity of APN, EGFR-TPK and MMP-2.

Key words： chlorogenic acid lung cancer A549 cell Aaoptosis reactive oxygen species epidermal growth factor receptor tyrosine kinase matrix metalloproteinase 2 aminopeptidase

收稿日期: 2016-03-21 出版日期: 2017-01-05

通讯作者: 2101749095@qq.com

引用本文:

田伟, 豆亚伟, 王宏涛, 朱建飞, 戴云罗, 向晖. 绿原酸诱导肺癌细胞凋亡及其机制研究[J]. 解放军预防医学杂志, 2016, 34(6): 854-857.
TIAN Wei, DOU Yawei, WANG Hongtao, ZHU Jianfei, DAI Yunluo, XIANG Hui. Effect of Chlorogenic Acid on Lung Cancer A549 Cell Apoptosis and Mechanism. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(6): 854-857.

链接本文:

http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I6/854

[1]	SIEGEL R,NAISHADHAM D,JEMAL A. Cancer statistics[J].CA Cancer J Clin,2013,63(1):11.
[2]	孙杰,牟晓燕,董雪丽.厄洛替尼和培美曲塞不同方式联合对肺腺癌A549细胞的作用[J].肿瘤,2013,33(12):1039.
[3]	黄逸生,杨衿记,黄玉娟,等.厄洛替尼二、三线治疗晚期非小细胞肺癌的临床研究[J].中国肺癌杂志,2009,12(5):412.
[4]	吴一龙,蒋国梁,陆舜,等.中国肺癌临床指南[M].北京:人民卫生出版社,2007.73.
[5]	刘馨, 陈晓群,李佳,等.绿原酸对MCF-7细胞增殖的影响及机制探讨[J].山东医药,2010,50(47):12.
[6]	孙秋艳,刘艳,汪茜,等.绿原酸对Lewis肺癌小鼠及人A549肺癌的实验研究[J].华西药学杂志,2010,25(5):357.
[7]	周红祖,徐丽霞,余惠旻,等.左金方、黄连、吴茱萸诱导人胃癌细胞SGC-7901凋亡的作用比较[J].药物分析杂志,2015,35(7):1160.
[8]	WANG Y J,HAN E,XING Q H,et al.Baicalein upregulates DDIT4 expression which mediates mTOR inhibition and growth inhibition in cancer cells[J].Cancer Letters,2015,358(2):170.
[9]	YING Z L,LU Q S,HAO F,et al.Design,synthesis,and preliminary aetivity studies of novelN-Cinnamoyl-L-aspartic acid derivatives as Aminopeptidase N/CD13 inhibitors[J].Bioorg Med Chem,2009,17(20):7398.
[10]	蒋超,蒙凌华,齐清,等.螺旋藻提取物对表皮生长因子受体酪氨酸激酶的抑制作用及诱导HL-60细胞凋亡[J].癌症,2000,19(12):1101.
[11]	谷丹,田俊秋.中药抗肿瘤作用机制的研究进展[J].黑龙江科技信息,2011,23(23):56.
[12]	杨家强,雷静,黎刚,等.肉桂酰氧基膦酸酯衍生物的合成与抗肿瘤活性[J].药学学报,2016,51(3):420.
[13]	李舒,王淑美,杨洋博君,等.益气除痰方降低肺癌Caspase 6、Lamin B1表达诱导细胞凋亡的机制[J].中成药,2016,38(3):683.
[14]	蒋洁敏,相芬芬,詹月萍,等.熊果酸增强人胃癌BGC-823细胞对紫杉醇敏感性的研究[J].世界中西医结合杂志,2016,11(2):185.
[15]	HUI T,WANG C,YE L.Comments on ethylenediaminetetraacetic acid contained in trypsin detaching cells for apoptosis detection[J].Stem Cells,2014,32(7):1993.
[16]	王芳,徐勤.中药挥发油诱导肿瘤细胞凋亡作用机制的研究进展[J].中国药房,2014,15(2):1424.
[17]	刘仪,王凯,王介非.氧化应激诱导细胞凋亡的机制[J].中华临床感染病杂志,2008,1(3):123.
[18]	SEPULVEDA M,GONANO L A,BACK T G,et al.Role of CaMKII and ROS in rapid pacing-induced apoptosis[J].Journal of Molecular & Cellular Cardiology,2013,63(2):135.
[19]	刘小敏,王晓娟,张杨,等.活性氧改变在PIG11高表达诱导人肝癌HepG-2细胞凋亡中的作用[J].肿瘤,2009,29(12):1116.
[20]	KIM S J,RABBANI Z N,DONG F,et al.Phosphorylated epidermal growth factor receptor and cyclooxygenase-2 expression in localized non small cell lung cancer[J].Med Oncol,2010,27(1):91.

No related articles found!

Viewed

Full text

Abstract

Cited

Shared

Discussed