高效液相色谱法同时测定畜禽废水中4种四环素类抗生素

全文: PDF(2856 KB)
输出: BibTeX | EndNote (RIS)

摘要目的利用高效液相色谱-二极管阵列检测器,建立同时检测畜禽废水中土霉素、四环素、金霉素、强力霉素的方法。方法选用Waters SunFire C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.5%三氟乙酸(TFA)水溶液为流动相梯度洗脱,流速1.2 ml/min,检测波长360 nm,高效液相色谱仪测定。结果土霉素、四环素、金霉素和强力霉素4种抗生素质量浓度为0.1 μg/ml~15.0 μg/ml时有良好的线性关系,4种抗生素的检出限均为50 ng/ml,方法回收率在83.82%~108.70%之间。结论高效液相色谱法方便、快速、准确,且重现性好,能够用于畜禽废水中四环素类抗生素的检测。

	服务

	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词 ：高效液相色谱法, 四环素类, 畜禽废水

Abstract：Objective To establish a method for determination of four tetracycline antibiotics in swine waste water by HPLC.Methods After filtration and acid-digestion, 20 μl samples were injected into an HPLC system. Acetonitrile and 0.5% TFA solution were applied in gradient elution using HPLC method. The total running time was 15 minutes.Results The standard curve of four compounds presented a good linearity(R₂>0.999) in the range of 0.1 to 15.0 μg/ml. The detection limits were 50 ng/ml. The recovery ranged from 83.82% to 108.70% with the relative standard deviation less than 9.30%.Conclusion The proposed method presents many advantages, such as quick speed, user-friendliness and reproducibility.

Key words： tetracycline antibiotics liquid chromatography swine wastewater

收稿日期: 2016-05-25 出版日期: 2017-01-05

基金资助:天津市科技计划项目(No.16YFZCNC00730)

通讯作者: gaozhx@163.com;zhouhytj@163.com

引用本文:

汤弘智, 陈风春, 王鸿英, 张永伟, 王永颖, 刘飞, 刘芳, 刘忠文, 高志贤, 周焕英. 高效液相色谱法同时测定畜禽废水中4种四环素类抗生素[J]. 解放军预防医学杂志, 2016, 34(6): 910-912.
TANG Hongzhi, CHEN Fengchun, WANG Hongying, ZHANG Yongwei,WANG Yongying, LIU Fei, LIU Fang, LIU Zhongwen, GAO Zhixian, ZHOU Huanying. Determination of Four Tetracycline Antibiotics in Swine Waste Water Using High-Performance Liquid Chromatography. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(6): 910-912.

链接本文:

http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I6/910

[1]	ZHANG Q Q, YING G G,PAN C G,et al.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis, multimedia modeling, and linkage to bacterial resistance[J].Enveronmental Science & Technology,2015,49:6772.
[2]	GB/T 5009.116-2003.畜、禽肉中土霉素、四环素、金霉素残留量的测定(高效液相色谱法) [S].2003.
[3]	佘永新,柳江英,吕晓玲,等.RP-HPLC法快速检测牛奶中7种四环素类药物残留量[J].食品科学,2009,30(12):157.
[4]	陆梅.高效液相色谱法测定水中的土霉素、金霉素、四环素残留[J].环境研究与检测,2009,22(3):39.
[5]	范志影,刘宏超.高效液相色谱法测定牛奶中的四环素类抗生素[J].中国乳品工业,2010,38(12):38.
[6]	方小丹,柯建明,陈振贺,等.固相萃取-超高效液相色谱法测定水中四环素类抗生素[J].生命科学仪器,2014,12(6):32.
[7]	SANDRA REVERTE,FRANCESC BORRULL,EVA POCURULL,et al.Determination of antibiotic compounds in water by solid-phase extraction-high-performance liquid chromatography-(electrospray) mass spectrometry[J].Journal of Chromatography A,2003,1010(2003):225.
[8]	胡冠九,王冰,孙成.高效液相色谱法测定环境水样中5种四环素类抗生素残留[J].环境化学,2007,26(1):106.
[9]	吴晓凤,郑嘉熹,魏源送,等.超高效液相色谱串联质谱法同时检测复杂基质中四环素类抗生素及其代谢产物[J].农业环境科学学报,2013,32(11):2293.
[10]	GB/T 21317-2007.动物源性食品中四环素类兽药残留量检测方法液相色谱-质谱/质谱法与高效液相色谱法 [S].2007.
[11]	BEN W W,QIANG Z M,ADAMS C,et al.Simultaneous determination of sulfonamides, tetracyclines and tiamulin in swine wastewater by solid-phase extraction and liquid chromatography-mass spectrometry[J].Journal of Chromatography A,2008,1202(2008):173.
[12]	李瑞萍,张艺,黄应平.环境水样中四环素类抗生素的检测技术[J].化学进展,2008,20(12):2075.
[13]	孙刚.畜禽粪便中四环素类抗生素检测分析及其在堆肥中的降解研究[D].合肥工业大学,2010.
[14]	刘小云,舒为群.水中抗生素污染现状及检测技术研究进展[J].中国卫生检验杂志,2005,15(8):1011.