青海省部分地区媒介及宿主动物体内携带伯氏疏螺旋体及基因型别的研究

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摘要目的了解青海省部分地区蜱及野鼠体内莱姆病螺旋体感染及其基因分型情况。方法采用布旗法采集蜱标本,采用夹夜法捕捉野鼠。经种类鉴定后,提取病原体DNA,应用巢式PCR 扩增鼠中莱姆病螺旋体5S-23S rRNA间隔区片段,对阳性产物进行限制性片段长度多态性(RFLP) 分析。结果检测3个区县3个蜱种共计618只蜱标本,其中共有3个蜱种97只标本检测阳性,总阳性率为15.69%。不同蜱种阳性率存在差别(χ²=9.68,P<0.01),其中以青海血蜱阳性率为最高,达到21.66%(47/217)。检测2个鼠种121只野鼠,其中12只检测阳性,总阳性率为9.91%,不同鼠种阳性率存在差别(χ²=4.64,P<0.05),以黄胸鼠的阳性率为最高,达到17.39%(8/46)。基因分型分析结果显示蜱及野鼠标本中莱姆病螺旋体为B. garinii 及B. afzelii基因型,其中B. garinii基因型占76.47%。结论青海省部分地区媒介及宿主动物体内存在莱姆病螺旋体,并至少有B. garinii 及B. afzelii两种基因型。

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关键词 ：莱姆病螺旋体, 基因型, 限制性片段长度多态性, 蜱, 野鼠

Abstract：Objective To investigate Borrelia burgdorferii infection in ticks and rodents captured in Qinghai Province and to identify their genotypes.Methods Ticks were collected by dragging a cloth over the vegetation while rodents were collected by snaps. After species identification, DNA was isolated from ticks and detected by nested PCR targeting 5S-23S rRNA intergenic spacer of Borrelia burgdorferii. Positive samples were further analysed by rest riction f ragment length polymorphism (RFLP) .Results A total of 618 adult ticks of 4 species, and 121 rodents of 2 species were tested for evidence of Borrelia burgdorferii by nested PCR.97 tick samples and 12 rodent samples showed positive results. The positive rate was different between different tick and rodent species, with Haemaphysalis qinghaiensis and Rattus tanezumi having the highest positive rates. The positive rate was 21.66% for Haemaphysalis qinghaiensis and 17.39% for Rattus tanezumi. RFLP analysis indicated that Borrelia burgdorferii in samples belonged to either B. garinii or B. afzelii.Conclusion Borrelia burgdorferii exists in Qinghai Province and is of at least two genotypes.

Key words： Borrelia burgdorferii genotype restriction of ragment length polymorphism (RFLP) tick rodents

收稿日期: 2016-07-09 出版日期: 2017-01-05

基金资助:全军后勤科研课题(No.CLZ15JA20,No.LZ13GY11)

引用本文:

张芳, 王伟, 李璐. 青海省部分地区媒介及宿主动物体内携带伯氏疏螺旋体及基因型别的研究[J]. 解放军预防医学杂志, 2016, 34(6): 799-802.
ZHANG Fang, WANG Wei, LI Lu. Borrelia Burgdorferii Infection and Genotypes in Ticks and Rodents Collected in Qinghai Province. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(6): 799-802.

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http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I6/799

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