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| Effects of Multiple Resistant Bacteria E. coli K12 (RP4) onAmmonia-oxidizing Bacteria in a Granular Sludge System |
| ZOU Wenci, ZHAO Tianyu, XUE Bin, YANG Dong, |
| Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China |
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Abstract Objective To investigate the effects of antibiotic resistance genes (ARGs) in E.coli K12(RP4) on the nitrification efficiency in biological wastewater treatment systems, so as to provide an evidence for controlling the risk of ARGs to wastewater treatment systems.Methods The donor strain E.coli K12(RP4) was inoculated in a lab-scale granular sequencing batch reactor (GSBR). Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative polymerase chain reaction (qPCR) approaches were used to explore the community structure diversity and abundance of ammonia-oxidizing bacteria (AOB) in microbial aggregates of various sizes.Results A decrease in removal efficiency of NH4+-N was observed after the inoculation of E.coli K12(RP4). For the granular sludge, as the particle size increased, the AOB community structure was more stable. In all sizes of sludge, Nitrosomonas was the dominant strain, and played an important role in the removal of NH4+-N. In the late stage, along with the ratio of AOB to total bacteria in flocculent sludge and less size granular promotion, the purification efficiency of NH4+-N returned to normal.Conclusions Multiple resistant bacteria E. coli K12 (RP4) affect the activity of ammonia-oxidizing bacteria, and then undermine nitrification performance of biological wastewater treatment systems.
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Published: 01 April 2016
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| Fund:国家自然科学基金青年项目(No.51408604);天津市应用基础与前沿技术研究计划(No.15JCZDJC40300) |
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2016, Vol. 34 
