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| Quercetin Promotes Axonal Growth and Functional Recovery after Spinal Cord Injury in Mice |
| DANG Yuanyuan, ZHANG Hongdian, YANG Yi, JI Xinchao, ZHANG Yan, LIU Ning, XU Ruxiang |
| Medical College of PLA, Beijing 100853, China |
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Abstract Objective To evaluate the effect of quercetin on axonal growth and functional recovery in mice after spinal cord injury.Methods Twenty-four C57BL/6 female mice were randomly and evenly divided into two groups. Complete compression injury at the spinal cords of T10 was induced in both groups . The mice in therapeutic group were administered with quercetin (50 mg/kg) while control group received the same dose of saline by intraperitoneal injection at 30min before injury, and immediately after injury, and then administered once a day from 1 to 10 days post-injury. The mice in control group received the same dose of saline with an equally long duration. Biotinylated dextran amine (BDA) was injected into the sensorimotor cortex at 14 days post-injury to trace the corticospinal tract (CST). Counterstaining was performed with glial fibrillary acidic protein (GFAP) to label the lesion boundary. The CST retraction and pullulation were observed under a fluorescent microscope. BMS was performed to assess motor functional recovery at 1, 3, 7, 14 and 28 days post-injury.Results Compared with control group, the ratio of total labeled sprouts/axons was significantly increased, the ratio of total labeled retraction bulbs/axons decreased,and the distance of the bundle to lesion border decreased after quercetin treatment at 28 days post-injury. The BMS scores were also significantly improved in therapeutic group (P<0.01).Conclusion Quercetin can promote axonal pullualtion, prevent axonal retraction and improve motor functional recovery after spinal cord injury in mice.
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Published: 14 September 2016
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2016, Vol. 34 
