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| Effects of Exogenous Dextranase and Sodium Fluoride on Formation of Multispecies Biofilms |
| QIU Yuanxin, FANG Shaowei, QIN Zongzhang, HU Tao |
| Tianjin Stomatological Hospital, Nankai University, Tianjin, 300041, China |
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Abstract Objective To investigate the effect of exogenous dextranase (Dex) combined with sodium fluoride (NaF) on the formation of multi-species biofilms.Methods A model of multi-species biofilms was established in vitro. The pH of inchoate biofilms was detected by placing the tip of a pH electrode into the matrix of the biofilms. The number of viable cells in inchoate biofilms was measured by colony count.Results 1. Dextranase had no effect on the pH of inchoate biofilms, but NaF at each tested concentration inhibited acid genesis of biofilms,which was not affected by Dex. 2. All the test agents significantly reduced viable cells on glass slides and 1U/mL ml Dex plus 80μg/mL ml NaF had synergistic effects on the formation of early biofilms.Conclusion Dextranase and sodium fluoride of low concentrations may have synergistic effects against the formation of multi-species biofilms.
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Published: 14 September 2016
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2016, Vol. 34 
