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| Impact of Porphyromonas Gingivalis on Nitric Oxide Synthase Expression in Pulmonary Epithelial Cells Infected with Influenza Virus |
| WANG Shengnan, LI Xin*, LI Yonggang, PAN Yaping |
| The Second Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China |
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Abstract Objective To observe the impact of Porphyromonas gingivalis on the expression of nitric oxide synthase in pulmonary epithelial cells infected with influenza virus .Methods Pulmonary epithelial cells were infected with 2 MOIs (multiplicity of infection) of H1N1 or different MOIs of Porphyromonas gingivalis before being divided into groups A-E. Nitric oxide (NO) production was assessed by measuring the accumulation of nitrite in culture supernatants. The level of inducible nitricoxide synthase (iNOS) was detected by Western blot .Results NO production increased in each group at 4h, 8h, 12h, 24h. The level of NO production in group E at 4h, 8h, 12h, 24h was 41.404 3.079, 78.945 8h, 1, 592.9838h, 12, 2360.660h, and 12h umol/L, respectively, and NO production at 8h, 12h, 24h was significantly higher than that in control group (P<0.05). The expression of iNOS in pulmonary epithelial cells infected with Influenza virus was more significantly promoted by Porphyromonas gingivalis at 12h, 24h, and than in control group (P<0.05).Conclusion Porphyromonas gingivalis may increase the expression of iNOS, thus promoting the synthesis of NO.
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Published: 14 September 2016
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2016, Vol. 34 
