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| Determination of Four Tetracycline Antibiotics in Swine Waste Water Using High-Performance Liquid Chromatography |
| TANG Hongzhi, CHEN Fengchun, WANG Hongying, ZHANG Yongwei,WANG Yongying, LIU Fei, LIU Fang, LIU Zhongwen, GAO Zhixian, ZHOU Huanying |
| Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Health and Environmental Medicine,Academy of Military Medical SciencesTianjin 300050,China |
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Abstract Objective To establish a method for determination of four tetracycline antibiotics in swine waste water by HPLC.Methods After filtration and acid-digestion, 20 μl samples were injected into an HPLC system. Acetonitrile and 0.5% TFA solution were applied in gradient elution using HPLC method. The total running time was 15 minutes.Results The standard curve of four compounds presented a good linearity(R2>0.999) in the range of 0.1 to 15.0 μg/ml. The detection limits were 50 ng/ml. The recovery ranged from 83.82% to 108.70% with the relative standard deviation less than 9.30%.Conclusion The proposed method presents many advantages, such as quick speed, user-friendliness and reproducibility.
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Received: 25 May 2016
Published: 05 January 2017
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| [1] |
ZHANG Q Q, YING G G,PAN C G,et al.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis, multimedia modeling, and linkage to bacterial resistance[J].Enveronmental Science & Technology,2015,49:6772.
|
| [2] |
GB/T 5009.116-2003.畜、禽肉中土霉素、四环素、金霉素残留量的测定(高效液相色谱法) [S].2003.
|
| [3] |
佘永新,柳江英,吕晓玲,等.RP-HPLC法快速检测牛奶中7种四环素类药物残留量[J].食品科学,2009,30(12):157.
|
| [4] |
陆梅.高效液相色谱法测定水中的土霉素、金霉素、四环素残留[J].环境研究与检测,2009,22(3):39.
|
| [5] |
范志影,刘宏超.高效液相色谱法测定牛奶中的四环素类抗生素[J].中国乳品工业,2010,38(12):38.
|
| [6] |
方小丹,柯建明,陈振贺,等.固相萃取-超高效液相色谱法测定水中四环素类抗生素[J].生命科学仪器,2014,12(6):32.
|
| [7] |
SANDRA REVERTE,FRANCESC BORRULL,EVA POCURULL,et al.Determination of antibiotic compounds in water by solid-phase extraction-high-performance liquid chromatography-(electrospray) mass spectrometry[J].Journal of Chromatography A,2003,1010(2003):225.
|
| [8] |
胡冠九,王冰,孙成.高效液相色谱法测定环境水样中5种四环素类抗生素残留[J].环境化学,2007,26(1):106.
|
| [9] |
吴晓凤,郑嘉熹,魏源送,等.超高效液相色谱串联质谱法同时检测复杂基质中四环素类抗生素及其代谢产物[J].农业环境科学学报,2013,32(11):2293.
|
| [10] |
GB/T 21317-2007.动物源性食品中四环素类兽药残留量检测方法 液相色谱-质谱/质谱法与高效液相色谱法 [S].2007.
|
| [11] |
BEN W W,QIANG Z M,ADAMS C,et al.Simultaneous determination of sulfonamides, tetracyclines and tiamulin in swine wastewater by solid-phase extraction and liquid chromatography-mass spectrometry[J].Journal of Chromatography A,2008,1202(2008):173.
|
| [12] |
李瑞萍,张艺,黄应平.环境水样中四环素类抗生素的检测技术[J].化学进展,2008,20(12):2075.
|
| [13] |
孙刚.畜禽粪便中四环素类抗生素检测分析及其在堆肥中的降解研究[D].合肥工业大学,2010.
|
| [14] |
刘小云,舒为群.水中抗生素污染现状及检测技术研究进展[J].中国卫生检验杂志,2005,15(8):1011.
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2016, Vol. 34 
