A PRELIMINARY STUDY ON THE EFFECT OF CYANIDIN-3,5-O-DIGLUCOSIDE ON 5-FLUOROURACIL INDUCED INTESTINAL MUCOSITIS IN MICE

FAN Jun-feng; LIU Jun-yang; QIN Xiao-hui; WANG Ke-ying; HUANG Chun-xiang; LIU Zhi-qiang; SONG Jia-le

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (2) : 146-154.

ORIGINAL ARTICLES

FAN Jun-feng¹, LIU Jun-yang², QIN Xiao-hui³, WANG Ke-ying², HUANG Chun-xiang², LIU Zhi-qiang³, SONG Jia-le^2,3

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Abstract

Objective To explore the effect of cyanidin-3,5-O-diglucoside (C35G) on 5-fluorouracil (5-FU)-induced intestinal mucositis in mice. Methods BALB/c male mice were randomly divided into normal group, model group, low-dose and high-dose C35G groups. Except for the normal group, mice in other groups were treated with 5-FU to induce a mouse model of intestinal mucositis for 4 days. Mice in the C35G low-dose and high-dose groups were intragastrically administrated with C35G (50 and 150 mg/kg) for 7 consecutive days. Finally, all mice were euthanized, and blood and colon tissues were collected. The levels of TNF-α, IL-1β and IL-10 in the serum were detected by enzyme-linked immunosorbent assay (ELISA). HE, PAS, and alcian blue staining kits were used to stain the colon tissue sections, and the pathological changes of colon tissues, the content of goblet cells and the secretion of mucin 2 were observed. Western blot was used to detect the expression levels of Wnt and c-Myc in colon tissus. Real-time quantitative PCR was used to detect the expression levels of tight junction proteingenes. Results Compared with the 5-FU group, the preventive intervention of C35G could significantly reduce the colonic mucosal damages in mice exposed to 5-FU. In addition, C35G intervention could also significantly reduce the levels of TNF-α and IL-1β in the serum (P<0.05), increase the level of IL-10 (P<0.01), and significantly increase the expression levels of Wnt and c-Myc in colon tissues. At the same time, the mRNA expression levels of tight junction proteins (Zo1, claudin1, claudin7, occludin) were significantly increased. Conclusion C35G is effective in ameliorating 5-FU-induced intestinal mucositis in mice.

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cyanidin-3 / 5-o-diglucoside / 5-fluorouracil / intestinal mucosal damage / inflammation

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FAN Jun-feng, LIU Jun-yang, QIN Xiao-hui, WANG Ke-ying, HUANG Chun-xiang, LIU Zhi-qiang, SONG Jia-le. A PRELIMINARY STUDY ON THE EFFECT OF CYANIDIN-3,5-O-DIGLUCOSIDE ON 5-FLUOROURACIL INDUCED INTESTINAL MUCOSITIS IN MICE[J]. Acta Nutrimenta Sinica. 2024, 46(2): 146-154

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