EFFECTS OF EARLY THIAMINE INTERVENTION ON DNA METHYLATION AND A&#x003b2; NEUROTOXICITY IN HIPPOCAMPAL NEURON CELLS

REN Gang; LIU Nai-rong; LI Jing; ZHAN Jie; LI Jing-hui

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (3) : 248-254.

ORIGINAL ARTICLES

EFFECTS OF EARLY THIAMINE INTERVENTION ON DNA METHYLATION AND Aβ NEUROTOXICITY IN HIPPOCAMPAL NEURON CELLS

REN Gang, LIU Nai-rong, LI Jing, ZHAN Jie, LI Jing-hui

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Abstract

Objective To explore the effects of early thiamine intervention on DNA methylation and intervention Aβ neurotoxicity in hippocampal neuron cells (HNCs). Methods HNCs from neonatal rats were cultured in vitro, and randomly divided into blank group, model group and low, medium and high dose groups of thiamine intervention. The HNCS were treated with different doses of thiamine for 3 days. The whole DNA methylation level of HNCs was determined by HPLC. The promoter methylation of CaMKIIα and GluR-2 genes was detected using MassARRAY. The cells were cultured in blank B27 medium until the cells matured. The effects of Aβ_1-42 on the proliferation rate (PR%) and apoptosis rate (AR%) of HNCS were studied. Results (1) The DNA methylation level of HNCs early exposed to thiamine treatment was lower than the blank group,and the medium dose group showed a significant difference (P<0.05). (2) Compared with the blank group, the methylation level of CaMKIIαpromoter CpG 3,11 in the low dose thiamine group decreased significantly (P<0.05),and the methylation level of GluR-2 promoter CpG5, 23 increased significantly (P<0.05). (3) The treatment of Aβ_1-42 caused a decreased cell proliferation rate and increased apoptosis rate. Compared with the model group, early thiamine intervention could increase proliferation rate. The high dose group was higher than the low and medium dose groups (P<0.05). Compared with the model group, early thiamine intervention could decrease apoptosis rate (P<0.05). There were no significant differences ( P>0.05) between different thiamine intervention groups. Conclusion Early thiamine intervention can regulate the methylation level of HNCs, and protect against the Aβneurotoxicity of mature hippocampal neuron cells through reducing the methylation level of CaMKIIαpromoter .

Key words

thiamine / DNA methylation level / Alzheimer’s disease

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REN Gang, LIU Nai-rong, LI Jing, ZHAN Jie, LI Jing-hui. EFFECTS OF EARLY THIAMINE INTERVENTION ON DNA METHYLATION AND Aβ NEUROTOXICITY IN HIPPOCAMPAL NEURON CELLS[J]. Acta Nutrimenta Sinica. 2024, 46(3): 248-254

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