EFFECTS OF PORTULACA OLERACEA L. POLYSACCHARIDES ON INTESTINAL FLORA STRUCTURE IN AGED RATS

HUANG Xiao-liu; LUO Hui; LONG Shu-jin; JIANG Hui; ZHANG Xing; YAN Hong-yu; HUNAG Yu-shan

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (3) : 276-282.

ORIGINAL ARTICLES

EFFECTS OF PORTULACA OLERACEA L. POLYSACCHARIDES ON INTESTINAL FLORA STRUCTURE IN AGED RATS

HUANG Xiao-liu¹, LUO Hui¹, LONG Shu-jin¹, JIANG Hui¹, ZHANG Xing¹, YAN Hong-yu², HUNAG Yu-shan³

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Abstract

Objective To investigate the effects of Portulaca oleracea polysaccharides (POP) on intestinal flora structure in aged rats. Methods Twenty elderly SD rats (21 months of age) were randomly divided into two groups with ten rats in each group. Control group (Con) rats were fed a normal diet, and intervention group (POP) rats were fed a diet supplemented with 1.0% POPs. All rats were fed for 15 consecutive weeks. At the end of the experiment, high-throughput sequencing technology (16S rRNA) was used to analyze the composition of intestinal flora. Results The results showed that there were 6087 identical operational taxonomic units (OTUs) in the two groups. Con group had 1025 characteristic OTUs, and POP group had 1131 characteristic OTUs. The intestinal flora of the two groups had similar alpha-diversity, but beta-diversity was significantly different between two groups. After ingestion of Portulaca oleracea polysaccharides, the relative abundances of Fusobacteria, Lactobacillus, Ruminiclostridium_9, Intestiniamonas, Faecalibacium, Desulfovibrio, [Bacteroides]_pectinophilus_group and Ruminiclostridium in the intestinal flora of aged rats decreased significantly, while the relative abundances of Parabacteroides, Ruminococcaceae_UCG-005 and Marvinbryantia increased significantly. The ingestion of Portulaca oleracea polysaccharides up-regulated the expression of genes related to cellular processes, metabolism and organismal systems, and down-regulated the expression of genes related to environmental information processing, human disease and genetic information processing in aged rats. Conclusion POP influences the structure of intestinal microflora in aged rats, and the Ruminococcaceae_UCG.005 and Parabacteroides are the main biomarkers.

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Portulaca oleracea L. polysaccharides / intestinal flora / aged rat

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HUANG Xiao-liu, LUO Hui, LONG Shu-jin, JIANG Hui, ZHANG Xing, YAN Hong-yu, HUNAG Yu-shan. EFFECTS OF PORTULACA OLERACEA L. POLYSACCHARIDES ON INTESTINAL FLORA STRUCTURE IN AGED RATS[J]. Acta Nutrimenta Sinica. 2024, 46(3): 276-282

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