EFFECT OF PARENTAL FOLIC ACID DEFICIENCY ON BRAIN URACIL MISINCORPORATION IN OFFSPRING RATS

ZHANG Guo-quan; REN Qing-han; CHANG Hong; HUANG Guo-wei; LI Zhen-shu; YAN Jing

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (4) : 348-355.

ORIGINAL ARTICLES

ZHANG Guo-quan¹, REN Qing-han¹, CHANG Hong^1,2, HUANG Guo-wei^1,2, LI Zhen-shu^1,2, YAN Jing^1,2

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Abstract

Objective To investigate the effect of parental folic acid deficiency on brain uracil misincorporation in offspring rats. Methods Forty-eight 6-week-old female rats and 24 6-week-old male rats were randomly divided into four groups: parental folic acid deficient group (D-D), maternal folic acid deficient and paternal folic acid normal group (D-N), maternal folic acid normal and paternal folic acid deficient group (N-D), and parental folic acid normal group (N-N). Each group had 12 female rats and 6 male rats. The offspring were fed the same diets of their mothers until postnatal day 100. For in vivo study, the deoxythymidine monophosphate (dTMP) and deoxyuridine monophosphate (dUMP) levels in brain tissue of offspring were detected by high-performance liquid chromatography at three different time points. For in vitro study, within 24 h after birth, neural stem cells (NSCs) were cultured for 7 days after being isolated from the hippocampus and striatum, and the dTMP and dUMP levels were measured. Results The level of dTMP in brain tissue was the lowest in the D-D group, followed by the D-N group and the N-D group, and the highest in the N-N group. The changes of dUMP level and dUMP/dTMP ratio were opposite to that of the dTMP. The dUMP level and dUMP/dTMP ratio in the brain tissue of the four groups increased with time, while the dTMP level decreased with time. The changes of dTMP, dUMP and dUMP/dTMP ratio of the NSCs cultured in vitro were consistent with those of brain tissue. Conclusion Parental folic acid deficiency increased uracil misincorporation in the brain tissue of offspring and the NSCs cultured in vitro in which the effect of parental folic acid deficiency was most significant, followed by maternal folic acid deficiency and paternal folic acid deficiency.

Key words

parental / folic acid / neural stem cells / deoxythymidine monophosphate / deoxyuridine monophosphate / uracil misincorporation

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ZHANG Guo-quan, REN Qing-han, CHANG Hong, HUANG Guo-wei, LI Zhen-shu, YAN Jing. EFFECT OF PARENTAL FOLIC ACID DEFICIENCY ON BRAIN URACIL MISINCORPORATION IN OFFSPRING RATS[J]. Acta Nutrimenta Sinica. 2024, 46(4): 348-355

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