EFFECT OF Bifidobacterium animalis Subsp. lactis (BPL1) ON BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS AND RELATED MECHANISMS

ZHANG Yi-chen; Kelsang Dekyi; ZHENG Lin-xi; XU Yu-han; ZHANG Wei; LI Hong-wei

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (6) : 574-582.

EFFECT OF Bifidobacterium animalis Subsp. lactis (BPL1) ON BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS AND RELATED MECHANISMS

ZHANG Yi-chen, Kelsang Dekyi, ZHENG Lin-xi, XU Yu-han, ZHANG Wei, LI Hong-wei

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Abstract

Objective To verify the effect of Bifidobacterium animalis subsp. lactis (BPL1) on blood pressure in spontaneously hypertensive rats (SHR) and to explore the possible mechanisms. Methods Fifty SHR rats were randomly divided into model control group (MC), captopril positive control group (PC), probiotic BPL1 high-dose [BPL1(H)], medium-dose [BPL1(M)] and low-dose groups [BPL1(L)] with 10 rats in each group. Ten Wistar-Kyoto (WKY) rats were selected as blank control group. Body mass, systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and heart rate were monitored during 30 days. After the experiment, the pathological sections of rat kidney were observed and the expression of renal angiotensin I converting enzyme (ACE) protein, biochemical and immunological related indexes, and the composition and changes of intestinal flora were analyzed. Results The SBP of the BPL1 dose groups was significantly lower than that of the model group (P<0.05). The DBP of the BPL1(M) and BPL1(H) groups was significantly lower than that in the model group, and the DBP of the BPL1 dose groups was close to the positive control group. However, the difference was not statistically significant. The MAP of the BPL1 dose groups was significantly lower than that of model group (P<0.05). Serum angiotensin II (AngⅡ )in the BPL1(L) and BPL1(H) groups was significantly lower than that in the model group (P<0.05). The level of aldosterone (ALD) in the BPL1 dose groups was significantly lower than that in the model group (P<0.05). The level of immunoglobulin A (IgA) in the BPL1(L) group was significantly higher than that in the model group (P<0.05). The level of tumor necrosis factor-α (TNF-α) in aorta after BPL1(M) treatment was significantly lower than that in the model group, and the level of interleukin-1β (IL-1β) in aorta after BPL1(M) treatment was significantly lower than that in the model group (P<0.05). The kidney expression of ACE protein in BPL1 dose groups was lower than that in the model group. The average F/B ratio in BPL1 dose groups was lower than that in the model group, and there was a downward trend with the increase of BPL1 dose. Conclusion Probiotic BPL1 has a remarkable antihypertensive effect by affecting renin-angiotensin-aldosterone system, inhibiting angiotensin I converting enzyme (ACE) activity, restricting Ang II conversion pathway, and restoring the balance of intestinal flora.

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Bifidobacterium animalis subsp. lactis (BPL1) / hypertension / angiotensin I converting enzyme / angiotensin II / aldosterone / intestinal flora

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ZHANG Yi-chen, Kelsang Dekyi, ZHENG Lin-xi, XU Yu-han, ZHANG Wei, LI Hong-wei. EFFECT OF Bifidobacterium animalis Subsp. lactis (BPL1) ON BLOOD PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RATS AND RELATED MECHANISMS[J]. Acta Nutrimenta Sinica. 2024, 46(6): 574-582

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