IN VITRO REDUCTION OF CHOLESTEROL AND IN VIVO ALLEVIATION OF FATTY LIVER IN HIGH-FAT DIET FED MICE BY THE BIFIDOBACTERIUM STRAINS ISOLATED FROM HEALTHY HUMANS

LI Jian-wei; TAN Ya-fang; YANG Rui-fu; MOU Rong; HAN Yan-ping

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (6) : 583-591.

IN VITRO REDUCTION OF CHOLESTEROL AND IN VIVO ALLEVIATION OF FATTY LIVER IN HIGH-FAT DIET FED MICE BY THE BIFIDOBACTERIUM STRAINS ISOLATED FROM HEALTHY HUMANS

LI Jian-wei^1,2, TAN Ya-fang², YANG Rui-fu^1,2, MOU Rong¹, HAN Yan-ping²

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Abstract

Objective To screen potential functional Bifidobacterium strains through in vitro lipid-lowering reduction experiments and evaluate their lipid-lowering effects in a high-fat diet fed mice. Methods Fourteen strains of Bifidobacterium were isolated from the feces of healthy volunteers. The well-accepted probiotics, Lactobacillus rhamnosus GG (LGG) were used as the control strain throughout this study. In vitro experiments were performed to detect cholesterol reduction rate, tolerance to acid and bile salt, and activity of bile salt hydrolase (BSH) of all tested strains. Those strains selected based on in vitro experiments were subject to the following animal experiments for verification in vivo. C57BL/6J mice were randomly divided into seven groups, including a normal diet control group (ND), a high-fat diet group (HFD), four high-fat diet intervention groups (gavaged with four Bifidobacterium strains,respectively, HFD+ Bifidobacterium), and a intervention control group (gavaged with LGG, HFD+LGG). Mice were fed normal diet or high-fat diet（60% kcal）and daily gavaged for 14 weeks with 0.2 ml PBS with or without 2×10⁸ CFU of bacterial strains. The changes of body weight were measured weekly, and the feces were collected once two weeks. All mice were sacrificed to collect bloods, livers, stools and other samples. Some parameters including fat index, serum biochemistry, hepatic lipids, liver pathology, and intestinal microbiota were analyzed. Results Based on the in vitro experiments, 4 of 14 Bifidobacterium strains（Bbr1、BA6、BL6、BB15）were selected for the animal experiments. The in vitro cholesterol degradation rates and BSH activity of the four Bifidobacterium strains were all higher than those of the LGG control strain (P<0.05). Besides, the four strains had a certain degree of acid and bile salt resistance activity. Animal experimental results showed that the body weights of the mice in the four Bifidobacterium intervention groups decreased during weeks 7-14 as compared with the HFD group. A significant reduction in hepatic cholesterol and hepatic triglyceride concentrations was observed after the intervention of BA6 and BL6. Liver pathological examination showed a decrease in the number of fatty vacuoles leading to the reduced liver pathology scores (P<0.05). Fecal microbiome analysis indicated that the compositions of the intestinal microbiota were changed after the gavage of BA6 and BL6. Interestingly, the abundance of harmful bacteria (e.g. Alloprevotella) was found decreased and that of beneficial bacteria (e.g. Lachnospiraceae) increased. Conclusion Based on the results of in vitro and in vivo experiments, two bacterial strains, Bifidobacterium adolescentis BA6 and Bifidobacterium longum BL6, play a potentail role in alleviating fatty liver, by improving organ’s indices, liver pathology, and gut microbiota composition. Functional experimental research data are further needed for the future development of probiotic products for lipid-lowering and fatty liver alleviation.

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Bifidobacterium / probiotics / cholesterol reduction / fatty liver / bile salt hydrolase

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LI Jian-wei, TAN Ya-fang, YANG Rui-fu, MOU Rong, HAN Yan-ping. IN VITRO REDUCTION OF CHOLESTEROL AND IN VIVO ALLEVIATION OF FATTY LIVER IN HIGH-FAT DIET FED MICE BY THE BIFIDOBACTERIUM STRAINS ISOLATED FROM HEALTHY HUMANS[J]. Acta Nutrimenta Sinica. 2024, 46(6): 583-591

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