PROANTHOCYANIDINS IMPROVE CELLULAR MITOCHONDRIAL DYSFUNCTION BY REGULATING MITOPHAGY IN SH-SY5Y CELLS

ZHANG Mei-rong; ZHANG Xiao-qiang; ZHANG Xiu-wen; DING Lan; YU Qi-jia

Acta Nutrimenta Sinica ›› 2024, Vol. 46 ›› Issue (6) : 599-605.

ORIGINAL ARTICLES

ZHANG Mei-rong^1,2, ZHANG Xiao-qiang^1,2, ZHANG Xiu-wen^1,2, DING Lan^1,2, YU Qi-jia^1,2

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Abstract

Objective To explore the mechanism of proanthocyanidins (PAC) in protecting against mitochondrial damage induced by N-methyl-4-phenylpyridinium iodide (MPP⁺) in SH-SY5Y cells. Methods SH-SY5Y cells were treated with MPP⁺ to establish an in vitro model of Parkinson's disease. The cells were randomly divided into control group, MPP+ group, PAC group, PAC + MPP⁺ group, 3-MA group, and 3-MA + PAC+ MPP⁺ group. Cell viability was assessed by the MTT method, ROS generation was measured by a chemical fluorescence method. JC-1 was used to detect mitochondrial membrane potential levels. Intracellular ATP content was determined by the molybdenum blue colorimetric method. The expression of mitochondrial autophagy-related proteins was detected by Western blot. Results Compared to the control group, treatment with MPP⁺ significantly reduced the viability of SH-SY5Y cells, and led to a significant increase in the accumulation of ROS, and significantly decreased ATP levels. Furthermore, compared to the control group, MPP+ treatment resulted in the decreases of cellular mitochondrial membrane potential, increased expression levels of the P62 protein, reduced expression levels of PHB2 proteins, and decreased ratio of LC3-II/LC3I. Treatment of SH-SY5Y cells with PAC was able to significantly reduce intracellular ROS accumulation induced by MPP⁺, increase ATP level, decrease the expression level of P62 protein, increase the expression level of PHB2 protein, and enhance the LC3-II/LC3I ratio. However, compared with the PAC+MPP⁺group, the pre-treatment with 3-MA significantly reduced the protective effects of PAC on MPP⁺-induced mitochondrial damage in SH-SY5Y cells. Conclusion PAC may alleviate mitochondrial dysfunction induced by MPP⁺ through the mitophagy.

Key words

proanthocyanidins / mitochondria / autophagy / SH-SY5Y cells

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ZHANG Mei-rong, ZHANG Xiao-qiang, ZHANG Xiu-wen, DING Lan, YU Qi-jia. PROANTHOCYANIDINS IMPROVE CELLULAR MITOCHONDRIAL DYSFUNCTION BY REGULATING MITOPHAGY IN SH-SY5Y CELLS[J]. Acta Nutrimenta Sinica. 2024, 46(6): 599-605

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