ANALYSIS OF THE ASSOCIATION BETWEEN SERUM FOLATE AND FIRST-EVER ISCHEMIC STROKE AND THE MEDIATION EFFECTS OF LNCRNA ANRIL

TONG Zong-hang; WANG Meng; LIU Yin-yue; YU Lan; Liu Sheng-jie; ZHANG Xu-mei

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (2) : 157-163.

ANALYSIS OF THE ASSOCIATION BETWEEN SERUM FOLATE AND FIRST-EVER ISCHEMIC STROKE AND THE MEDIATION EFFECTS OF LNCRNA ANRIL

TONG Zong-hang¹, WANG Meng¹, LIU Yin-yue¹, YU Lan¹, Liu Sheng-jie², ZHANG Xu-mei¹

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Abstract

Objective To explore the association between serum folate and first-onset ischemic stroke (IS), and to investigate the mediating effects of long non-coding RNA ANRIL (lncRNA ANRIL). Methods A case-control study was conducted by descriptive analysis, correlation analysis, and mediation analysis on 112 first-ever ischemic stroke patients and 142 healthy controls. The percentile Bootstrap method was used to test the significance of the mediating effects. Results After adjusting for factors such as age, gender, BMI, education level, smoking, alcohol consumption, hypertension, diabetes, and hypercholesterolemia, lncRNA ANRIL levels were found to be associated with an increased risk of ischemic stroke (lncRNA ANRIL≥1.64: OR, 5.52; 95% CI: 3.07-10.12). Lower serum folate levels were associated with an increased risk of ischemic stroke (folate <7.00 nmol/L: OR, 2.12; 95% CI: 1.15-3.90). Serum folate levels were negatively correlated with lncRNA ANRIL levels (β=–0.26; 95% CI: –0.44-–0.08). Mediation analysis showed that lncRNA ANRIL had a partial mediating effect on the association between serum folate and IS, accounting for 51.27% of the total effect. Conclusion High serum folate level significantly reduces the risk of first-ever IS, while lncRNA ANRIL not only increases the risk of IS but also plays a partial mediating role in the association between serum folate and IS.

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ischemic stroke / lncRNA ANRIL / folate / mediating effect

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TONG Zong-hang, WANG Meng, LIU Yin-yue, YU Lan, Liu Sheng-jie, ZHANG Xu-mei. ANALYSIS OF THE ASSOCIATION BETWEEN SERUM FOLATE AND FIRST-EVER ISCHEMIC STROKE AND THE MEDIATION EFFECTS OF LNCRNA ANRIL[J]. Acta Nutrimenta Sinica. 2025, 47(2): 157-163

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