CURCUMIN IMPROVES SLEEP DISORDER IN MICE EXPOSED TO THE CONFINED ENVIRONMENT

ZHANG Chen-yang; BIAN Xiang-yu; WANG Feng; YU Li-xia; GUO Chang-jiang; YAO Zhan-xin

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (2) : 185-190.

ORIGINAL ARTICLES

CURCUMIN IMPROVES SLEEP DISORDER IN MICE EXPOSED TO THE CONFINED ENVIRONMENT

ZHANG Chen-yang^1,2, BIAN Xiang-yu², WANG Feng², YU Li-xia², GUO Chang-jiang^1,2, YAO Zhan-xin²

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Abstract

Objective To investigate the effect of curcumin on sleep disorder in mice exposed to confined environmental conditions. Methods Thirty-six male KM mice were randomly assigned to the control group (CON), model group (M), and curcumin group (J). The CON group was kept under the usual conditions in the animal house. The M group and J group were restrained for 4h in a biorhythm box under the altered light condition. The light condition in one cycle was as follows: the light time was opposite to that of the CON group in the first 3d, and returned to the normal from the 4th to the 6th. Group J was given 100 mg/ (kg·bw) curcumin by gavage daily, group con and M was given an equal amount of saline.The experiment lasted for 30 d. The mice were injected intraperitoneally with sodium pentobarbital to induce sleep, and the sleep latency and duration were recorded. Finally mice were sacrificed and the serum and hypothalamus tissues were taken. The contents of cortisol (CORT), corticotropin-releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) in serum and 5-hydroxytryptamine (5-HT) and dopamine (DA) in hypothalamus tissues were measured by enzyme-linked immunosorbent assay (ELISA). The expressions of hypothalamus-associated clock genes were detected by RT-qPCR. Results The results of the sodium pentobarbital-induced sleep test showed that curcumin significantly shortened sleep latency (P<0.01) and prolonged sleep time (P<0.01). Meanwhile curcumin significantly reduced serum CORT, ACTH, and CRH levels compared with the group M (P<0.01, P<0.05). In the hypothalamus-related neurotransmitters, 5-HT content was significantly increased (P<0.01) and DA content was significantly decreased (P < 0.05) in group J compared with group M. Curcumin could regulate the expressions of biorhythmic genes (Clock, Bmal1, Per1, Per2, Per3, Cry1, Cry2, Rev-erbα/β) in the hypothalamus. Conclusion Curcumin improves sleep disorder by modulating the expression of related biorhythmic genes and altering related serum hormones and hypothalamic neurotransmitters.

Key words

confined environment / sleep disorder / curcumin / hypothalamic neurotransmitters / serum hormone / biological rhythm genes

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ZHANG Chen-yang, BIAN Xiang-yu, WANG Feng, YU Li-xia, GUO Chang-jiang, YAO Zhan-xin. CURCUMIN IMPROVES SLEEP DISORDER IN MICE EXPOSED TO THE CONFINED ENVIRONMENT[J]. Acta Nutrimenta Sinica. 2025, 47(2): 185-190

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