LACTOBACILLUS BULGARICUS PROMOTE CONJUGATIVE TRANSFER OF DRUG-RESISTANT PLASMIDS IN VITRO

TIAN Xue; WANG Ping-yu; JIN Min; YANG Xuan-xuan; YANG Dong; LI Jun-wen

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (3) : 292-299.

ORIGINAL ARTICLES

LACTOBACILLUS BULGARICUS PROMOTE CONJUGATIVE TRANSFER OF DRUG-RESISTANT PLASMIDS IN VITRO

TIAN Xue^1,2, WANG Ping-yu¹, JIN Min², YANG Xuan-xuan^1,2, YANG Dong², LI Jun-wen²

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Abstract

Objective To explore the effect of Lactobacillus bulgaricus GIM1.155 on the conjugative transfer of multi-drug resistant plasmid (RP4). Methods The donor bacterium was Escherichia coli MG1655 K12 containing multi-drug resistant plasmid RP4 with resistance against tetracycline, kanamycin and ampicillin, and the recipient bacterium was Klebsiella pneumoniae with meropenem resistance. The ratio of donor and recipient bacteria was kept at 1:1, and the splicing system was 10mL. Finally, the donor and recipient bacteria and splices were screened out using resistant plates containing different antibiotics, and the frequency of conjugative transfer was calculated. The effects of donor and recipient volume gradients (10⁶, 10⁷, 10⁸, 10⁹ CFUml), as well as GIM1.155 volume gradients (10⁷, 10⁸, 10⁹, 10¹⁰ CFU/ml), temperature (4, 25, 37℃), time (1, 2, 4, 8, 12, 24, 36, 48h) and pH (2, 4, 6, 7, 8, 9) on the conjugative transfer of RP4 plasmid were investigated. Results Lactobacillus bulgaricus GIM1.155 could promote the conjugative transfer of RP4 plasmid from E. coli MG1655 K12 to K. pneumoniae, and the promotion effect was dependent on the amount of the donor-recipient bacterium, the amount of the bacterium of GIM1.155, as well as the temperature, time and pH of conjugative transfer. Conclusion Lactobacillus bulgaricus GIM1.155 can promote the conjugative transfer of multi-drug resistant plasmid RP4 under certain conditions.

Key words

Lactobacillus bulgaricus / conjugative transfer / multidrug resistance gene / Escherichia coli / Klebsiella pneumoniae

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TIAN Xue, WANG Ping-yu, JIN Min, YANG Xuan-xuan, YANG Dong, LI Jun-wen. LACTOBACILLUS BULGARICUS PROMOTE CONJUGATIVE TRANSFER OF DRUG-RESISTANT PLASMIDS IN VITRO[J]. Acta Nutrimenta Sinica. 2025, 47(3): 292-299

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