EFFECTS OF HIGH PROTEIN DIET ON URINARY METABOLITE PROFILES IN FOOD RESTRICTED RATS

SU Nan; LIU Jin; AN Peng; WU Jin; BAI Jia-lei; WANG Yong-hui

PDF(3470 KB)

Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (4) : 340-346.

ORIGINAL ARTICLES

EFFECTS OF HIGH PROTEIN DIET ON URINARY METABOLITE PROFILES IN FOOD RESTRICTED RATS

SU Nan¹, LIU Jin², AN Peng³, WU Jin¹, BAI Jia-lei¹, WANG Yong-hui¹

Author information +

History +

Abstract

Objective To investigate the effects of high-protein diet on urinary metabolome in rats with food restriction. Methods Twenty-four male Wistar rats were randomly divided into three groups (8 per group). The normal control group received 15% casein diet and water ad-libitum. The food restriction group was pair-fed the 15% casein diet with 30% food intake of the normal control. The protein supplemented group was pair-fed 30% casein diet with the 30% food intake of the normal control. Urine samples were collected 2 weeks after pair feeding. Nuclear magnetic resonance spectrometer was used to investigate the changes of urinary metabolite profiles. Results Both food restriction and protein supplemented groups significantly altered the urinary metabolites. Food restriction led to a decrease in the levels of citric acid, α-ketoglutaric acid, N-acetyl glycoprotein and an increase in the level of taurine in the urine, while the levels of citric acid, α-ketoglutaric acid, and N-acetyl glycoprotein were higher in the protein supplemented group than in the food restriction group, and the level of taurine was also higher than in the food restriction group. Conclusion A high-protein diet is helpful in improving metabolic disorders in the food restricted rats.

Key words

high protein diet / food restriction / nuclear magnetic resonance / urinary metabolites / rats

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SU Nan, LIU Jin, AN Peng, WU Jin, BAI Jia-lei, WANG Yong-hui. EFFECTS OF HIGH PROTEIN DIET ON URINARY METABOLITE PROFILES IN FOOD RESTRICTED RATS[J]. Acta Nutrimenta Sinica. 2025, 47(4): 340-346

References

[1] 王竟夷,刘晋,钱平,等. 应急食品研究现状及发展趋势[J]. 中国农学通报, 2022, 38: 132–137.
[2] Schonfeldt HC, Hall NG.Dietary protein quality and malnutrition in Africa[J]. Br J Nutr, 2012, 108: S69–S76.
[3] 王永辉,李培兵,于晓明,等. 高蛋白膳食对限食大鼠肠免疫功能的保护作用[J]. 解放军预防医学杂志, 2010,28:242–244.
[4] 王永辉,李培兵,南文考,等. 高蛋白膳食对限食大鼠血清和肠粘膜氨基酸谱的影响[J]. 营养学报,2010, 32:438–441.
[5] Suzuki M, Katamine S, Tatsumi S.Exercise-induced enhancement of lipid peroxide metabolism in tissue and their transference into the brain in rat[J]. J Nutr Sci Vitaminol,1983, 29:141–146.
[6] Smith LL.Similarities and differences regarding acute anorexia nervosa and semi-starvation: does behavioral thermoregulation play a central role[J]. Front Behav Neurosci, 2023, 17: 1243572.
[7] Singh U, Alsuhaymi S, Al-Nemi R, et al. Compound-specific 1D¹H NMR pulse sequence selection for metabolomics analyses.[J]. ACS Omega, 2023, 26: 23651–23663.
[8] Trygg J, Holmes E, Lundstedt T.Chemometrics in metabonomics[J]. J Proteome Res, 2007, 6: 469–479.
[9] Clarke CJ, Haselden JN.Metabolic profiling as a tool for understanding mechanisms of toxicity[J]. Toxicol Pathol, 2008, 36: 140–147.
[10] Bel Lassen P, Belda E, Prifti E, et al. Protein supplementation during an energy-restricted diet induces visceral fat loss and gut microbiota amino acid metabolism activation: a randomized trial[J]. Sci Rep, 2021, 1: 15620.
[11] Tong X, Zhang DQ, Charney N, et al. DDB1-Mediated CRY1 degradation promotes FOXO1-driven gluconeogenesis in liver[J]. Diabetes, 2017, 12: 2571–2582.
[12] Puranik S, Sahu PP, Srivastava PS, et al. NAC proteins: regulation and role in stress tolerance[J]. Trends Plant Sci, 2012, 21: 166–170.
[13] Ueki I, Stipanuk MH.3T3-L1 adipocytes and rat adipose tissue have a high capacity for taurine synthesis by the cysteine dioxygenase/cysteine sulfinate decarboxylase and cysteamine dioxygenase pathways[J]. J Nutr, 2009, 2: 207–214.
[14] Ribeiro R, Bonfleur ML, Batista TM, et al. Regulation of glucose and lipid metabolism by the pancreatic and extra-pancreatic actions of taurine[J]. Amino Acids, 2018, 11: 1511–1524.
[15] El Idrissi A, El Hilali F, Rotondo S, et al. Effects of taurine supplementation on neuronal excitability and glucose homeostasis[J]. Adv Exp Med Biol, 2017, 975(Pt 1): 271–279.
[16] Carneiro EM, Latorraca MQ, Araujo E, et al.Taurine supplementation modulates glucose homeostasis and islet function[J]. J Nutr Biochem, 2009, 20: 503–511.