Objective To explore the effects of manganese supplementation on lung inflammation in obese asthmatic mice exposed to ovalbumin (OVA) and to provide new insights for clinical treatment. Methods Sixty male Kunming mice were randomly divided into five groups: control group (CON), obesity group (HF), asthma group (OVA), obese asthma group (HF+OVA), and obese asthmatic manganese intervention group (Mn, hereinafter referred to as the Mn intervention group), with 12 mice in each group. Groups HF, HF+OVA, and Mn were fed a high-fat diet for 12 weeks, and the other two groups were fed a control diet. Mice in the OVA, HF+OVA, and Mn intervention groups were sensitized by intraperitoneal injection of 200 μl of 0.05 mg/ml OVA sensitization solution on the first day of the 9th, 10th, and 11th weeks. On the 12th week, mice were treated by nasal drip 50 μl of 0.05 mg/ml OVA saline solution for 3 consecutive days to induce asthma. During the feeding period of high-fat diet, the Mn intervention group received 1 mg/kg MnCl2 intranasally every two days to induce asthma. After the last nasal drip exposure, asthmatic behavioral changes were observed and airway hyperresponsiveness was measured. Body weight was measured every fortnight during the modeling period, body composition was measured after 12 weeks. Hematoxylin-eosin staining was performed to observe the pathological changes in lung tissues. White blood cells in mouse bronchoalveolar lavage fluid (BALF) were counted and classified. The Mn contents in the serum and lung tissues were detected, and the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in BALF were analyzed. Results Compared with the CON group, the mice in the HF group gained 12.4% in body weight, while the HF+OVA group gained 23.2%. The body fat ratios increased by 46.1% and 67.7% in the HF and HF+OVA groups, indicating the obesity model was successfully constructed. The mice in the OVA and HF+OVA groups showed symptoms such as nose scratching and nodding breathing, with increased airway hyperresponsiveness, indicating that the asthma model was established. Significant pathological changes were observed in the lung tissue of asthma mice, with an increased number of inflammatory cells and elevated levels of IL-6 and TNF-α. These symptoms were more pronounced in HF+OVA mice (P0.05). Compared with the HF+OVA group, mice in the Mn intervention group showed a 17.9% reduction in body weight and a 27.4% reduction in body fat ratio. Meanwhile, asthma symptoms were mitigated, airway hyperresponsiveness decreased, and pathological damages to lung tissues reduced. The number of inflammatory cells was decreased, and the levels of IL-6 and TNF-α levels declined (P0.05). Conclusion Mn supplementation protects against inflammatory responses in the lungs of obese asthmatic mice, and its mechanism is related to inhibited inflammatory responses.
Key words
obesity /
asthma /
manganese /
inflammatory response
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