APPLE POLYPHENOLS EXTRACT ALLEVIATES ABNORMALITES OF HEPATIC LIPID METABOLISM IN DSS-INDUCED ACUTE ULCERATIVE COLITIS MICE

LI Xin-ran; QIAN Qing-fan; MA Jie-yu; LUAN Wen-xue; LI Xin-li

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (5) : 477-485.

APPLE POLYPHENOLS EXTRACT ALLEVIATES ABNORMALITES OF HEPATIC LIPID METABOLISM IN DSS-INDUCED ACUTE ULCERATIVE COLITIS MICE

LI Xin-ran, QIAN Qing-fan, MA Jie-yu, LUAN Wen-xue, LI Xin-li

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Abstract

Objective To investigate the effects of apple polyphenol extract (APE) on the changes of hepatic lipid metabolism induced by dextran sodium sulfate (DSS) in mice with acute ulcerative colitis (UC) and the underlying mechanisms. Methods Thirty male C57BL/6 mice aged 6-8 weeks were randomly divided into three groups, namely control (CON), DSS model (DSS) and DSS model treated with APE (AD). All mice were fed the chow diet. Mice in CON group were given distilled water for 6 weeks. For mice in the DSS group, distilled water was provided for the first 5 weeks and 3% DSS (m/v) solution in drinking water was given for the last week. For mice in the AD group, distilled water was provided provided for the first 5 weeks and 3% DSS (m/v) solution in drinking water was given for the last week, simultaneously with 500 mg/(kg·bw) APE gavage for 6 weeks. After the intervention, blood samples from the angular vein were obtained, while colon and liver were collected for subsequent histological examination. Quantitative real time polymerase chain reaction (RT-qPCR) was employed to detect the mRNA expression of genes related to hepatic lipid metabolism. Results APE intervention significantly reduced DAI scores in UC mice, ameliorated colonic pathological damages, diminished hepatic lipid deposition and inflammatory cell infiltration, reduced NAS, and along with significantly reduced serum levels of TG, AST, ALT, as well as levels of TNF-α, IL-6 and IL-1β. Further mRNA analysis showed that compared with the CON group, the mRNA expression levels of the fatty acid oxidation-related genes Pparα, fatty acid uptake gene Cd36, lipolysis gene Atgl, cholesterol reverse transporter genes Abcg8, bile acid synthesis genes Cyp7a1 and the key regulator of hepatic lipid metabolism Sirt1 were significantly decreased (P<0.05). The mRNA levels of Acc, Tgr5, ‌proinflammatory cytokine Tnf-α and Il-6 were significantly increased (P<0.05). Compared with the DSS group, APE intervention significantly increased the mRNA expression levels of Sirt1, Sr-b1 and Lxrα (P<0.05),reduced the mRNA expression levels of Acc, Tgr5 and Tnf-α (P<0.05). Conclusion APE improves acute UC-associated hepatic lipid metabolism abnormalities by regulating the mRNA levels of hepatic lipid metabolism-related genes.

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apple polyphenols extract / dextran sodium sulfate / acute ulcerative colitis / liver / lipid metabolism

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LI Xin-ran, QIAN Qing-fan, MA Jie-yu, LUAN Wen-xue, LI Xin-li. APPLE POLYPHENOLS EXTRACT ALLEVIATES ABNORMALITES OF HEPATIC LIPID METABOLISM IN DSS-INDUCED ACUTE ULCERATIVE COLITIS MICE[J]. Acta Nutrimenta Sinica. 2025, 47(5): 477-485

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