Objective To study the effects of hydroethanolic extract of Cydonia oblonga Mill. (HECO) on fatty liver induced by high fat diet in mice and its potential mechanism. Methods Twenty-four male C57BL/6J mice were randomly assigned to the control group (CON), high-fat diet group (HFD), and HECO intervention group (HECO). The latter two groups were fed with a high-fat diet. HECO group were additionally treated with 200 mg/(kg·d) HECO by oral gavage. The intervention lasted for 12 weeks, followed by liver histology examination, measurements of serum and hepatic lipid levels, hepatic function, as well as the oxidative stress biomarkers, inflammatory cytokines, lipid metabolism genes expression, NF-kB p65 phosphorylation and Nrf2 nuclear translocation. Results Compared with the HFD group, HECO significantly decreased body weight, inhibited hepatic lipid deposition, increased high-density lipoprotein cholesterol level and reduced triglycerides, total cholesterol, low-density lipoprotein cholesterol, aspartate aminotransferase and alanine aminotransferase levels in serum (P<0.05). HECO also significantly decreased hepatic TG, TC and free fatty acid levels, increased the activities of glutathione peroxidase, superoxide dismutase and catalase, and elevated glutathione level. Meanwhile, hepatic MDA, interleukin-1β, interleukin-6, and tumor necrosis factor-α levels were reduced. The expressions of lipogenesis genes, Srebp1c, Fasn, Acc1, and Scd1 were downregulated, and the expressions of fatty acid transport and β oxidation genes, Cpt1α, Fgf21, Cd36 and Fabp1 were upregulated. Additionally, HECO significantly inhibited the phosphorylation of NF-κB and enhanced the nuclear translocation of Nrf2 (P<0.05). Conclusion HECO is effective in preventing metabolic dysfunction-associated fatty liver disease possibly by maintaining hepatic redox status, inhibiting inflammation and improving lipid metabolism.
Key words
fatty liver /
extract of Cydonia oblonga Mill. /
lipid metabolism /
oxidative stress /
inflammation
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