PROTECTIVE EFFECTS OF NERVONIC ACID ON LIPOPOLYSACCHARIDE-INDUCED INFLAMMATORY INJURY IN INTESTINAL EPITHELIAL CELLS

ZHANG Ming-yu; MO Shu-lan; YAN Chuan-zhi; DENG Pei-lin; KE Zhi-zhu; SONG Jia-le

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (6) : 581-593.

ORIGINAL ARTICLES

PROTECTIVE EFFECTS OF NERVONIC ACID ON LIPOPOLYSACCHARIDE-INDUCED INFLAMMATORY INJURY IN INTESTINAL EPITHELIAL CELLS

ZHANG Ming-yu¹, MO Shu-lan¹, YAN Chuan-zhi², DENG Pei-lin¹, KE Zhi-zhu¹, SONG Jia-le^1,3

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Abstract

Objective To investigate the protective effects of nervonic acid (NA) on lipopolysaccharide (LPS)-induced intestinal epithelial barrier dysfunction in vitro. Methods The cells were divided into three groups: normal group, LPS-treated group, and three LPS+NA groups (5, 10, and 20 μmol/L). An intestinal injury cell model was established by treating Caco-2 and HT-29 cells with LPS for 24 hours. After co-treatment with NA and LPS, cells were cultured for an additional 24 hours, and viability was evaluated using the CCK-8 assay. Immunofluorescence staining was used to detect NF-κB p65 nuclear translocation as well as the distribution of tight junction (TJ) proteins (Claudin-1, Claudin-7) and mucins (MUC2, MUC5AC). Additionally, mRNA or protein expression levels of inflammatory factors (Tnfα, Il1β), TJ proteins (ZO-1, Claudin-1, Claudin-7, Occludin), mucins (MUC2, MUC5AC), and adhesion proteins (Alpi, Desmosome, Jama, Ecadherin) were detected by Western blotting or PCR analysis in the cells. Results NA significantly improved the viability of LPS-treated Caco-2 and HT-29 cells. NA treatment inhibited the nuclear translocation of NF-κB p65 in Caco-2 cells. Additionally, it reversed the downregulation of Claudin-1 and Claudin-7 expressions in Caco-2 cells, as well as the downregulation of MUC2 and MUC5AC expressions in HT-29 cells after LPS exposure. Moreover, NA treatment also upregulated the mRNA expressions of Zo1, Claudin1 and Occludin in both Caco-2 and HT-29 cells exposed to LPS. Furthermore, NA treatment decreased the mRNA expressions of Tnfα, Il1β and increased the expressions of Claudin-1 and Claudin-7 in Caco-2 cells exposed to LPS. It also elevated the mRNA expressions of mucins (Muc2, Muc3, Muc5ac) and adhesion proteins (Alpi, Desmosome, Jama, Ecadherin) in HT-29 cells exposed to LPS. Conclusion NA alleviates LPS-induced damage in Caco-2 and HT-29 cells by reducing inflammatory factor levels, upregulating TJ protein expressions, and ultimately restoring intestinal epithelial barrier function.

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nervonic acid / lipopolysaccharide / inflammatory injury / intestinal cell

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ZHANG Ming-yu, MO Shu-lan, YAN Chuan-zhi, DENG Pei-lin, KE Zhi-zhu, SONG Jia-le. PROTECTIVE EFFECTS OF NERVONIC ACID ON LIPOPOLYSACCHARIDE-INDUCED INFLAMMATORY INJURY IN INTESTINAL EPITHELIAL CELLS[J]. Acta Nutrimenta Sinica. 2025, 47(6): 581-593

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