GANODERMA LUCIDUM POLYSACCHARIDES AMELIORATE RADIATION-INDUCED INTESTINAL INJURY IN MICE BY IMPROVING GUT MICROECOLOGY

CHAI Xu-xia; JIN Lu; TAO Meng-di; GUO Chang-jiang; YAO Zhan-xin; CHANG Hong

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Acta Nutrimenta Sinica ›› 2025, Vol. 47 ›› Issue (6) : 602-611.

GANODERMA LUCIDUM POLYSACCHARIDES AMELIORATE RADIATION-INDUCED INTESTINAL INJURY IN MICE BY IMPROVING GUT MICROECOLOGY

CHAI Xu-xia^1,2, JIN Lu², TAO Meng-di², GUO Chang-jiang², YAO Zhan-xin², CHANG Hong¹

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Abstract

Objective To investigate the effects of Ganoderma lucidum polysaccharides on intestinal microecology to reduce radiation-induced small intestine damage in mice. Methods C57BL/6J male mice were randomly divided into three groups: blank control group, radiation model group and Ganoderma lucidum polysaccharides group, with 15 mice in each group. Mice were given orally with saline or 150 mg/(kg·bw) Ganoderma lucidum polysaccharides once a day for 28 days. On the 15th day, mice in the radiation model group and the Ganoderma lucidum polysaccharides group were given a single whole-body 6 Gy dose of irradiation. On the 27th day, fresh feces samples were collected. On the 28th day, the samples of sera and small intestine tissue were taken after anesthesia. The radiation damage of small intestine was assessed by HE staining, ELISA method, and Western blot analysis. 16S rRNA high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS) were used to analyze the changes of intestinal microbiota and short-chain fatty acids (SCFAs). Pearson correlation analysis was used to explore the associations among microbiota, metabolites and intestinal parameters. Results Compared with the radiation model group, Ganoderma lucidum polysaccharides intervention significantly improved pathological changes of intestinal mucosa caused by radiation, reduced serum D-lactate (D-LA) content, diamine oxidase (DAO) activity, and lipopolysaccharide (LPS) content (P<0.05), and up-regulated the expression of zona occludens 1(ZO-1), claudin1 and mucin 2 (MUC2) (P<0.05）. Meanwhile, Ganoderma lucidum polysaccharides also relieved oxidative stress and inflammation by reducing malondialdehyde (MDA) content, increasing superoxide dismutase (SOD) and catalase (CAT) activities, decreasing the levels of pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and increasing anti-inflammatory cytokine interleukin-10 (IL-10) level (P<0.05). Ganoderma lucidum polysaccharides significantly improved radiation-induced dysbacteriosis and increased the contents of acetic acid and butyric acid in feces (P<0.05). Correlation analysis showed that Alistipes, Odoribacter, Enterorhabdus and other bacteria were correlated with the improvements in oxidative stress, inflammation and intestinal barrier function, while other genera Eubacterium_ xylanophilum_ group, Mucispirillum, Faecalibaculum, Turicibacter, Eubacterium_siraeum_group, etc. were related to the levels of short-chain fatty acids. Conclusion Ganoderma lucidum polysaccharides reduce radiation-induced small intestine damage possibly by improving the intestinal microecology.

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Ganoderma lucidum polysaccharides / radiation-induced injury / gut microbiota / intestinal barrier / short-chain fatty acids

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CHAI Xu-xia, JIN Lu, TAO Meng-di, GUO Chang-jiang, YAO Zhan-xin, CHANG Hong. GANODERMA LUCIDUM POLYSACCHARIDES AMELIORATE RADIATION-INDUCED INTESTINAL INJURY IN MICE BY IMPROVING GUT MICROECOLOGY[J]. Acta Nutrimenta Sinica. 2025, 47(6): 602-611

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