Objective To determine serum 25-hydroxyvitamin D levels in children aged 6-8 years in Henan and Guizhou Provinces, and analyzed the intergroup differences as well as their associated influencing factors. Methods Children aged 6-8 years from Henan and Guizhou were enrolled from YYB intervention cohort in 2018.Ultra-performance liquid chromatography-mass spectrometry with isotope internal standard method was used to quantitatively detect serum 25-(OH)D₃ and 25-(OH)D₂ levels, and their correlations with region, milk intake and outdoor activity duration were analyzed. Results The overall vitamin D status of the study children was good. The serum 25-(OH)D concentrations were 43.7(35.3,52.6) ng/mL in Fuquan (Guizhou), 33.4(27.0,40.1) ng/mL in Guiding (Guizhou), 28.7(24.0,32.8) ng/mL in Songxian (Henan), and 30.4(24.1,37.4) ng/mL in Ruyang (Henan), with 6.8% insufficient. Guizhou children had significantly higher vitamin D levels than Henan children (P<0.0001). Milk intake and outdoor activity duration were significantly correlated with serum vitamin D concentrations (P=0.0048, P<0.0001). Conclusion The vitamin D status in children was influenced by several factors, such as milk intake and outdoor activity duration.
Key words
school-aged children /
25-hydroxyvitamin D /
UPLC-MS /
serum /
outdoor activity time
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