Objective To investigate the effects of manganese supplementation on the obesity induced by high-fat diet in mice. Methods Forty-eight male C57BL/6 mice were randomly divided into four groups: control group (control), manganese group (Mn), high-fat diet group (HFD), and HFD plus manganese supplementation group (HFD+Mn), with 12 mice in each group. Groups HFD and HFD+Mn were fed a high-fat diet (60% fat, 20% protein and 20% carbohydrate) for 12 weeks, and the other two groups were fed a control diet (10% fat, 20% protein and 70% carbohydrate). The Mn and the HFD+Mn groups were received 1.43 mg/kg Mn orally during the feeding period. Body weight and body composition were detected. After 12 weeks, the mice were anesthesized and specimens were collected. The contents of manganese in blood and biochemical markers in serum were examined. The pathological change of brown adipose tissue (BAT) was observed. The oxidative stress were evaluated in brown adipose tissue (BAT) by analyzing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). The effects of Mn supplementation and HFD on BAT mitochondrial function were assessed by detecting the mitochondrial ROS (mtROS), ATP level and mitochondrial membrane potential. Results Compared to the control group, body weight, fat mass, and serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) of the HFD group were notably elevated (P<0.05), while the level of high-density lipoprotein cholesterol (HDL-C) significantly reduced (P<0.05). HE staining revealed that the morphology of BAT was abnormal in the HFD group. The oxidative stress in BAT was obviously increased, manifested as increased contents of ROS and MDA. Increase of mitochondrial ROS (mtROS), and decrease of ATP level and mitochondrial membrane potential were also observed in BAT (P<0.05). Mn supplementation reduced the degree of obesity in mice (P<0.05), improved serum lipid levels (P<0.05), and alleviated the abnormal morphology of BAT. Mn supplementation also decreased oxidative stress and improved mitochondrial function in BAT (P<0.05). Conclusion Mn supplementation can ameliorate obesity induced by high-fat diet and improve mitochondrial function of BAT in mice.
Key words
obesity /
manganese /
brown adipose tissue /
mitochondrial function /
mice
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