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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (5): 471-475.doi: 10.12047/j.cjap.5494.2017.113

• 研究论文 • 上一篇    下一篇

TAK1基因沉默对TNF-a诱导的滑膜细胞IL-6、IL-8表达的影响

莫选荣1, 谢江文2, 吕国菊2, 柯于平1, 罗心静1   

  1. 1. 台州学院医学院基础部, 浙江 台州 318000;
    2. 宁波市鄞州区第二医院心内科, 浙江 宁波 315000
  • 收稿日期:2016-09-09 修回日期:2017-05-15 出版日期:2017-09-28 发布日期:2018-06-19
  • 通讯作者: 罗心静,Tel:15068647955;E-mail:luoxjing@126.com E-mail:luoxjing@126.com
  • 基金资助:
    国家自然科学基金面上项目(81373139);浙江省自然科学基金(LY16H100001);浙江省医药卫生科研项目(2015KYA206)

Effects of TAK gene silencing on the expressions of IL-6 and IL-8 induced by TNF-α in fibroblast-like synoviocytes

MO Xuan-rong1, XIE Jiang-wen2, LV Guo-ju2, KE Yu-ping1, LUO Xin-jing1   

  1. 1. Department of Basic Medical Sciences, School of Medicine of Taizhou University, Taizhou 318000;
    2. Department of Cardiology, Yingzhou District Second people's Hospital, Ningbo 315000, China
  • Received:2016-09-09 Revised:2017-05-15 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    国家自然科学基金面上项目(81373139);浙江省自然科学基金(LY16H100001);浙江省医药卫生科研项目(2015KYA206)

摘要: 目的:观察转化生长因子β激活激酶1(TAK1)基因沉默对肿瘤坏死因子a (TNF-a)诱导的滑膜细胞中促炎介质白介素-6(IL-6)和白介素-8(IL-8)表达的影响,以探讨TAK1在类风湿关节炎(RA)发病中的作用。方法:采取脂质体转染方法将TAK1特异性的小干扰RNA (siRNA)和阴性对照RNA (scRNA)导入类风湿关节炎的滑膜成纤维细胞株MH7A细胞,然后分别用20 ng/ml TNF-a刺激后,检测细胞内IL-6、IL-8 mRNA的表达和培养上清中IL-6和IL-8分泌情况以及p38、ERK、JNK、p65磷酸化的水平和抑制性蛋白IκBα的变化情况。结果:siRNA-TAK1转染72 h后滑膜细胞中TAK mRNA和蛋白表达的平均抑制率分别为75%和55%。siRNA-TAK1转染下调TNF-a诱导状态下IL-6和IL-8的表达,并能抑制p38、JNK、p65磷酸化和增加IκBα水平。结论:TAK1基因沉默能抑制TNF-a诱导的滑膜细胞IL-6、IL-8表达,可能与其抑制JNK和p38MAPK的活化及NF-κB的活化有关。

关键词: 类风湿关节炎, 滑膜细胞, 炎症介质, TAK1

Abstract: Objective: To investigate the effects of silencing transforming growth factor-β activating kinase 1 (TAK1)on the expressions of IL-6 and IL-8 induced by TNF-α in fibroblast-like synoviocytes, and to explore the role of TAK1 in rheumatoid arthritis (RA).Methods: The synthesized TAK1 siRNA and scrambled siRNA (ScRNA) were transferred into cultured RA fibroblast-like synoviocyte line MH7A by lipofectamine. The expressions of the pro-inflammatory mediator IL-6 and IL-8 and the levels of phospho-P38(p-P38), phospho-C-Jun NH2-terminal kinase(p-JNK), phospho-extracellular signal-regulated kinase(p-ERK), phospho-p65(p-p65) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha(IκBa) were examined.Results: Silencing of TAK was demonstrated in synoviocytes transfected by TAK siRNA. TAK1 silencing markedly attenuated the expression of IL-6 and IL-8 in the presence of TNF-α. TAK1 silencing inhibited the activation of p38 and JNK MAPK. TAK1 silencing also inhibited activation of nuclear factor-κB (NF-κB).Conclusion: TAK1 silencing attenuated the expression of IL-6 and IL-8 in synoviocytes induced by TNF-α via inhibiting the activation of p38, JNK MAPK and NF-κB.

Key words: rheumatoid arthritis, synoviocytes, inflammatory mediators, TAK1

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