APPswe/PS1dE9转基因小鼠小脑内突触素及BDNF/Trk-B蛋白表达的变化

doi:10.12047/j.cjap.5601.2017.116

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (6): 488-492.doi: 10.12047/j.cjap.5601.2017.116

APPswe/PS1dE9转基因小鼠小脑内突触素及BDNF/Trk-B蛋白表达的变化

董雪帆, 王婷, 李甜, 焦娟娟, 曲雪松, 祁金顺, 杨威

山西医科大学生理学系, 细胞生理学省部共建教育部重点实验室, 太原 030001

收稿日期:2016-11-28 修回日期:2017-05-23 出版日期:2017-11-28 发布日期:2018-06-19
基金资助:
山西省回国留学人员科研资助项目（2016060）

Expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice

DONG Xue-fan, WANG Ting, LI Tian, JIAO Juan-juan, QU Xue-song, QI Jin-shun, YANG Wei

Department of Physiology, Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, China

Received:2016-11-28 Revised:2017-05-23 Online:2017-11-28 Published:2018-06-19
Contact: 杨威,Tel:13293910485;E-mail:vividmail@163.com E-mail:vividmail@163.com
Supported by:
山西省回国留学人员科研资助项目（2016060）

摘要/Abstract

摘要： 目的：观察APP/PS1转基因小鼠小脑突触素及BDNF/Trk-B蛋白表达变化。方法：选用9月龄APP/PS1雄鼠（n1）和同窝对照野生型WT雄鼠（n2）。采用Western blot （n1=6；n2=6）、免疫组化（n1=4；n2=4）两种方式定量、定位测定小脑组织功能活性依赖蛋白突触素、脑源性神经营养因子（BDNF）和其高亲和力受体（Trk-B）的蛋白表达。用透射电镜观察小脑皮质突触超微结构变化（n1=2；n2=2）。结果：与WT组相比，APP/PS1组小脑皮质内突触素、BDNF/Trk-B表达明显减少；突触间隙增宽，突触后致密区变薄，密度降低。结论：APP/PS1小鼠小脑皮质中突触素、BDNF/Trk-B蛋白含量均明显降低，突触超微结构也发生明显改变，提示AD小脑突触数量及形态变化可能与BDNF合成及释放减少有关。

关键词: APP/PS1转基因小鼠, 小脑, 突触素, BDNF/Trk-B, 突触

Abstract: Objective: To observe the expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice.Methods: The healthy 9-month old APP/PS1 male mice (n1) and the same wild type male mice(n2) were divided into two groups, APP/PS1 group and wild-type(WT) group. The expressions of synaptophysin and brain-derived neurotrophic factor/tyrosine kinase B (BDNF/Trk-B) in cerebellum were determined by Western blot (n1=6; n2=6) and immunohistochemical(n1=4; n2=4).The possible synaptic changes in APP/PS1 group were observed by using electron microscopy.Results: Compared with WT group, the expressions of synaptophsin and BDNF/Trk-B in cerebellum were decreased in APP/PS1 group. Increased width of the synaptic cleft and decreased thickness of postsynaptic density were also observed.Conclusion: In APP/PS1 group, expressions of synaptophsin and BDNF/Trk-B in cerebellum were decreased; changes in ultrastructure of synapses seemed to be widespread alterations. These findings suggest a possible association between expression of BDNF/TrkB and synaptic plasticity in AD cerebellum.

Key words: APP/PS1 transgenic mouse, cerebellum, synaptophysin, BDNF, Trk-B, synapse

董雪帆, 王婷, 李甜, 焦娟娟, 曲雪松, 祁金顺, 杨威. APPswe/PS1dE9转基因小鼠小脑内突触素及BDNF/Trk-B蛋白表达的变化[J]. 中国应用生理学杂志, 2017, 33(6): 488-492.

DONG Xue-fan, WANG Ting, LI Tian, JIAO Juan-juan, QU Xue-song, QI Jin-shun, YANG Wei. Expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice[J]. CJAP, 2017, 33(6): 488-492.

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APPswe/PS1dE9转基因小鼠小脑内突触素及BDNF/Trk-B蛋白表达的变化

Expressions of synaptophysin and BDNF/Trk-B in cerebellum of APPswe/PS1dE9 transgenic mice

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