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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (6): 550-554.doi: 10.13459/j.cnki.cjap.2016.06.014

• 研究论文 • 上一篇    下一篇

5-HT1B受体亚型对小脑顶核介导的运动行为的影响

高伟1, 王楠2, 乔虎2   

  1. 1. 西安理工大学体育部, 陕西 西安 710048;
    2. 西安交通大学医学部, 陕西 西安 710061
  • 出版日期:2016-11-28 发布日期:2018-06-19
  • 通讯作者: 高伟,Tel:029-82312959;E-mail:59073939@qq.com E-mail:59073939@qq.com
  • 基金资助:
    国家自然科学基金项目(30900544)

The effects of 5-HT1B receptor subtypes on motor behaviors mediated by cerebellar fastigial nucleus

GAO Wei1, WANG Nan2, QIAO Hu2   

  1. 1. Department of Physical Education, Xi'an University of Technology, Xi'an 710048;;
    2. Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China
  • Online:2016-11-28 Published:2018-06-19
  • Supported by:
    国家自然科学基金项目(30900544)

摘要: 目的:探讨5-羟色胺(5-HT)能神经系统在经小脑顶核介导的运动行为中的作用。方法:采用大鼠离体脑片膜片钳及大鼠走步机的行为学测试方法。结果:阻断5-HT1B受体能够增强小脑顶核兴奋性突触传递,行为学试验中给予5-HT及5-HT1B受体阻断剂SB224289,发现注射5-HT到小脑顶核后,大鼠在Rota-rod走步机上的持续时间显著延长,而给予其阻断剂SB224289后,能够反转此作用。结论:5-HT很可能通过5-HT1B受体抑制顶核神经元的兴奋性突触传递从而调节小脑核团神经元环路的活动,继而影响小脑的最终输出,实现对小脑顶核介导的运动平衡和协调能力的调控。

关键词: 5-HT, 5-HT1B受体, 小脑顶核, 兴奋性突触后电流, 大鼠, 膜片钳

Abstract: Objective: To investigate the effects of the serotonergic nervous system on motor behaviors mediated by cerebellar fastigial nucleus (FN). Methods: In this experiment, the main methods are whole-cell patch clamp recording and Rota-rod test. Results: We found that the excitatory synaptic transmission was enhanced in the cerebella FN after blocking 5-hydroxytryptamine,(5-HT)receptor. Microinjection of 5-HT into FNs remarkably promoted motor performances on Rota-rod, which could be reversed by microinjection of 5-HT receptor antagonist SB224289. Conclusion: These results suggest that the 5-HT can suppress cerebellar FN excitatory synaptic transmission via 5-HT1B receptors, thereby modulate the activity of cerebellar nuclear neurons circuitry, and subsequently influence the cerebellum-mediated ongoing motor balance and coordination.

Key words: 5-HT, 5-HT1B receptor, fastigial nucleus (FN), excitatory post-synaptic currents, rat, patch clamp

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