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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (5): 466-470.doi: 10.13459/j.cnki.cjap.2016.05.021

• 研究论文 • 上一篇    下一篇

缺血/再灌注时豚鼠左心室流出道自律组织电活动的改变及药物干预效应

赵兰平1, 薛淑芳1, 陈彦静2, 王雪芳1, 陈立锋1, 范玉宏3, 张祥宇1   

  1. 1. 河北北方学院生理教研室, 张家口 075000;
    2. 中国中医科学院中医基础理论研究所, 北京 100700;
    3. 河北北方学院第一临床医学院, 张家口 075000
  • 收稿日期:2015-08-03 修回日期:2016-05-18 出版日期:2016-09-28 发布日期:2018-06-20
  • 通讯作者: 赵兰平,Tel:0313-4029313;E-mail:zhaolp2009@126.com E-mail:zhaolp2009@126.com
  • 基金资助:
    河北省卫生厅重点科技研究计划项目(20130038);河北北方学院创新人才培育项目(CXRC1324)

Electrophysiological effects of ischemia/reperfusion (I/R) on pacemaker cells in guinea-pig left ventricular outflow tract and the effects of antiarrhythmic drugs treatment

ZHAO Lan-ping1, XUE Shu-fang1, CHEN Yan-jing2, WANG Xue-fang1, CHEN Li-feng1, FAN Yu-hong3, ZHANG Xiang-yu1   

  1. 1. Department of Physiology, Hebei North University, Zhangjiakou 075000;
    2. Institute of Basic Theories, China Academy of Chinese Medical Sciences, Beijing 100700;
    3. The First Clinical Medical College, Hebei North University, Zhangjiakou 075000, China
  • Received:2015-08-03 Revised:2016-05-18 Online:2016-09-28 Published:2018-06-20
  • Supported by:
    河北省卫生厅重点科技研究计划项目(20130038);河北北方学院创新人才培育项目(CXRC1324)

摘要: 目的:研究缺血/再灌注(I/R)时豚鼠离体左心室流出道自律组织电活动的改变及其药物干预效应。方法:采用标准玻璃微电极细胞内电位记录技术,记录豚鼠离体左心室流出道标本的自发慢反应电位,观测模拟I/R时该电位的改变,以及临床上常用的抗心律失常药物灌流标本时对I/R电生理效应的干预作用。观测指标:4相自动除极速度(VDD)、自发放电频率(RPF)、最大舒张电位(MDP)、0相最大除极速度(Vmax)、动作电位幅度(APA)、复极50%和90%时间(APD50和APD90)。结果:①与对照组相比,I 10 min组VDD和RPF明显减慢(P<0.05),Vmax加快(P<0.01),APA增大(P<0.01)。与I 10 min组和对照组相比,R 2 min组VDD和RPF明显加快(P<0.01),且在R至5 min过程中可出现明显节律不齐,MDP绝对值明显增大(P<0.05),Vmax与对照组相比明显加快(P<0.05),APA与I 10 min组相比明显下降(P<0.05),但仍显著高于对照组(P<0.05),APD50和APD90显著缩短(P<0.01)。R 15 min组自发慢反应电位各项指标逐渐恢复至对照组水平。②与I 10min/R 2 min组相比,1 μmol/L利多卡因、10 μmol/L普罗帕酮、1 μmol/L胺碘酮、1 μmol/L维拉帕米、50 μmol/L腺苷和10 μmol/L硝普钠均可明显改善R过程中VDD和RPF的改变以及由此引起的节律不齐。结论:I/R可触发左心室流出道自律组织电活动异常,这种效应在应用不同的抗心律失常药物灌流时可显著恢复。

关键词: 缺血/再灌注, 左心室流出道, 自律组织, 药物干预, 豚鼠

Abstract: Objective: To investigate the electrophysiological effects of ischemia/reperfusion (I/R) on the spontaneous slow response action potentials in guinea-pig left ventricular outflow tract and the effects of antiarrhythmic drugs onI/R. Methods: The action potentials of pacemaker cells in guinea-pig left ventricular outflow tract were recorded by conventional intracellular microelectrode technique. The influences of ischemia(I) 10 min, reperfusion(R) 2 min, and R 15min on the spontaneous slow response potentials were investigated. The effects of lidocaine, propafenone, amiodarone, verapamil, adenosine, and sodium nitroprusside (SNP) on I/R were also studied. Electrophysiological parameters were examined:velocity of diastolic depolarization(VDD), rate of pacemaker firing(RPF), maximal diastolic potential(MDP), maximal rate of depolarization(Vmax), amplitude of action potential(APA), 50% and 90% of duration of action potential(APD50 and APD90). Results: ①In I 10 min group, the values of VDD, RPF, Vmax and APA were decreased markedly compared with control group (P<0.05, P<0.01).In R 2 min group, VDD and RPF were increased significantly(P<0.01), MDP was increased notably(P<0.05), APD50 and APD90 were shortened significantly compared with I 10 min and control group. Vmax was increased markedly vs control group(P<0.05). APA was decreased notably vs I 10 min group (P<0.05), but was increased markedly vs control group(P<0.05). In R 15 min group, the action potentials recovered gradually to the levels of control group. ② Compared with I 10 min/R 2 min group, 1 μmol/L lidocaine, 10 μmol/L propafenone, 1 μmol/L amiodarone, 1 μmol/L verapamil, 50 μmol/L adenosine, and 10 μmol/L SNP decreased VDD and RPF significantly. Conclusion: I/R injury can trigger abnormal spontaneous activities of guinea-pig left ventricular outflow tract.The electrophysiological effects of I/R injury on left ventricular outflow tract can be treated by antiarrhythmic drugs.

Key words: ischemia/reperfusion, left ventricular outflow tract, electrophysiological effect, drug treatment, guinea-pig

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