关键词: 血必净, 内毒素, 急性肺损伤, 肿瘤坏死因子α, 白细胞介素10, 高迁移率族蛋白-1

Abstract: Objective: To investigate the protective effects of xuebijing (Traditional Chinese Medicine)on the acute lung injury in rats. Methods: Sixty Wistar rats were randomly divided into normal control group,model group,dexamethasone (10 mg/kg) group, xuebijing low-dose,medium-dose and high-dose groups(5, 10, 15 ml/kg),10 rats in each group. The acute lung injury model was induced by intraperitoneal injection of lipopolysaccharide 5 mg/kg. They were given relevant medicines intraperitoneally after modeling, once a day, for consecutive 7 days. Normal control group and acute lung injury(ALI) model group were given the equal bulk of saline by tail vein. 7 days after treatment, the arterial blood samples were collected for detecting the arterial oxygen tension (PaO₂) and the serum levels of malondialdehyde (MDA) and superoxide dismutase (SOD). Pulmonary tissue samples were obtained for the measurement of lung index(LI), wet/dry weight ratio(W/D), moisture content of lung[(W-D)/W] and the expressions of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and high mobility group protein B1(HMGB1). Results: Compared with normal control group,the levels of LI,W/D,(W-D)/W and PaO₂ and the expressions of TNF-α, HMGB1 protein and the serum level of MDA in model group were increased,while the protein expression of IL-10 and the serum level of SOD were decreased;there was statistical significance(P < 0.01). Compared with model group,the levels of LI,W/D,moisture content of lung and the protein expressions of TNF-α and HMGB1 and the serum level of MDA in xuebijing groups were decreased significantly,while PaO₂ and the protein expression of IL-10 and the serum level of SOD were increased, there was statistical significance (P < 0.01). The effects in xuebi-jing high-dose group were better than those in xuebijing middle-dose and low-dose groups (P < 0.01, P < 0.05), Conclusion: Xuebijing could alleviate lipopolysaccharide-induced ALI,and its pharmacological mechanism may be related to down-regulation the protein expressions of TNF-α and HMGB1 and the serum level of MDA and up-regulation the protein expression of IL-10 and the serum level of SOD and the high-dose is more effective.

Key words: Xuebijing injection, lipopolysaccharide, acute lung injury, TNF-α, IL-10, HMGB1