不同功率的激光刺激对小鼠C2C12成肌细胞耗氧率水平的影响及机制

doi:10.12047/j.cjap.5667.2018.095

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (5): 418-421.doi: 10.12047/j.cjap.5667.2018.095

不同功率的激光刺激对小鼠C₂C₁₂成肌细胞耗氧率水平的影响及机制

冯钰, 程泽鹏, 孙鹏, 史仍飞

上海体育学院运动科学学院, 上海 200438

收稿日期:2018-01-03 修回日期:2018-09-25 出版日期:2018-09-28 发布日期:2019-02-21
通讯作者: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
基金资助:
史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn

Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei

Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China

Received:2018-01-03 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
Contact: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
Supported by:
史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn

摘要/Abstract

摘要： 目的：探讨不同功率的低强度650 nm激光刺激对C₂C₁₂成肌细胞耗氧率水平和相关蛋白的影响及其机制。方法：以体外培养的C₂C₁₂小鼠成肌细胞作为实验对象，以4×10⁵个/孔接种于牵张6孔板中，采用输出功率5 mW，波长650 nm的二极管激光进行单次刺激，激光照射剂量分别0 J/cm²（0 min）、0.4 J/cm²（12.8 min）、0.8 J/cm²（25.6 min）。实验结束后，采用耗氧率试剂盒（Luxcel Biosciences）检测细胞耗氧率；提取细胞总蛋白，采用Western blot技术检测成肌调节因子（MyoD）、过氧化物酶体增殖活化受体γ共激活因子1α（PGC-1α）、雷帕霉素靶蛋白和磷酸化蛋白（p-mTOR/mTOR）表达。结果：与对照组相比，低剂量组细胞氧化耗氧率结果、MyoD、PGC-1α蛋白表达显著增加（P<0.05），高剂量组MyoD、PGC-1α蛋白表达显著增加（P<0.05），p-mTOR/mTOR蛋白显著降低（P<0.05）。结论：较低剂量（0.4 J/cm²）的650 nm低强度激光增强了细胞氧化功能水平，并对细胞分化相关蛋白有一定影响。其机制可能与适宜的激光刺激影响PGC-1α蛋白的表达，进而影响线粒体氧化呼吸有关。

关键词: 低强度激光, 耗氧率, PGC-1α, 小鼠C₂C₁₂成肌细胞

Abstract: Objective: To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in C₂C₁₂ myoblasts following different doses. Methods: C₂C₁₂ cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm². Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot. Results: Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(P<0.05). MyoD and PGC-1α protein expressions were also increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(P<0.05). Conclusion: 650 nm laser irradiation that dose is 0.4 J/cm² enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.

Key words: laser irradiation, oxygen consumption rate, PGC-1α, C₂C₁₂ cells

冯钰, 程泽鹏, 孙鹏, 史仍飞. 不同功率的激光刺激对小鼠C₂C₁₂成肌细胞耗氧率水平的影响及机制[J]. 中国应用生理学杂志, 2018, 34(5): 418-421.

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei. Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice[J]. CJAP, 2018, 34(5): 418-421.

参考文献

[1] Shefer G, Ben-Dov N, Halevy O, et al. Primary myogenic cells see the light:improved survival of transplanted myogenic cells following low energy laser irradiation[J]. Lasers Surg Med, 2008, 40(1):38-45.
[2] Leal-Junior EC, De Almeida P, Tomazoni SS, et al. Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression[J]. PLoS One, 2014, 9(3):e89453.
[3] Shefer G, Oron U, Irintchev A, et al. Skeletal muscle cell activation by low-energy laser irradiation:a role for the MAPK/ERK pathway[J]. J Cell Physiol, 2001, 187(1):73-80.
[4] Shefer G, Partridge TA, Heslop L, et al. Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells[J]. J Cell Sci, 2002, 115(Pt 7):1461-1469.
[5] Alexsandra da Silva Neto Trajano L, da Silva CL, de Carvalho SN, et al. Cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser[J]. Lasers Med Sci, 2016, 31(5):841-848.
[6] Wang X, Huang N, Yang M, et al. FTO is required for myogenesis by positively regulating mTOR-PGC-1α pathway-mediated mitochondria biogenesis[J]. Cell Death Dis, 2017, 8(3):e2702.
[7] Cunningham JT, Rodgers JT, Arlow DH, et al. mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex[J]. Nature, 2007, 450(7170):736-740.
[8] Martins F, Renno AC, Oliveira F, et al. Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats[J]. Acta Cir Bras, 2015, 30(2):94-99.
[9] Rudnicki MA, Schnegelsberg PN, Stead RH, et al. MyoD or Myf-5 is required for the formation of skeletal muscle[J]. Cell, 1993, 75(7):1351-1359.
[10] Mcintosh LM, Garrett KL, Megeney L, et al. Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin- and MyoD-deficient muscles[J]. Anat Rec, 1998, 252(2):311-324.
[11] Alves AN, Ribeiro BG, Fernandes KP, et al. Comparative effects of low-level laser therapy pre-and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair[J]. Lasers Med Sci, 2016, 31(4):679-685.
[12] Brunelli RM, Rodrigues NC, Ribeiro DA, et al. The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion[J]. Lasers Med Sci, 2014, 29(1):91-96.
[13] Macedo AB, Moraes LH, Mizobuti DS, et al. Low-level laser therapy (LLLT) in dystrophin-deficient muscle cells:Effects on regeneration capacity, inflammation response and oxidative stress[J]. PLoS One, 2015, 10(6):e0128567.
[14] Houreld NN, Masha RT, Abrahamse H. Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells[J]. Laser Surg Med, 2012, 44(5):429-434.
[15] Hay N, Sonenberg N. Upstream and downstream of mTOR[J]. Genes Dev, 2004, 18(16):1926-1945.
[16] Martin DE, Hall MN. The expanding TOR signaling network[J]. Curr Opin Cell Biol, 2005, 17(2):158-166.
[17] Sperandio FF, Giudice FS, Correa L, et al. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway[J]. J Biophotonics, 2013, 6(10):839-847.

不同功率的激光刺激对小鼠C₂C₁₂成肌细胞耗氧率水平的影响及机制

Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice

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