Effects of laser stimulation with different power on oxygen consumption rate of C2C12 myoblasts in mice

doi:10.12047/j.cjap.5667.2018.095

CJAP ›› 2018, Vol. 34 ›› Issue (5): 418-421.doi: 10.12047/j.cjap.5667.2018.095

Previous Articles Next Articles

Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei

Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China

Received:2018-01-03 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
Contact: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
Supported by:
史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn

Abstract

Abstract: Objective: To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in C₂C₁₂ myoblasts following different doses. Methods: C₂C₁₂ cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm². Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot. Results: Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(P<0.05). MyoD and PGC-1α protein expressions were also increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(P<0.05). Conclusion: 650 nm laser irradiation that dose is 0.4 J/cm² enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.

Key words: laser irradiation, oxygen consumption rate, PGC-1α, C₂C₁₂ cells

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei. Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice[J]. CJAP, 2018, 34(5): 418-421.

References

[1] Shefer G, Ben-Dov N, Halevy O, et al. Primary myogenic cells see the light:improved survival of transplanted myogenic cells following low energy laser irradiation[J]. Lasers Surg Med, 2008, 40(1):38-45.
[2] Leal-Junior EC, De Almeida P, Tomazoni SS, et al. Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression[J]. PLoS One, 2014, 9(3):e89453.
[3] Shefer G, Oron U, Irintchev A, et al. Skeletal muscle cell activation by low-energy laser irradiation:a role for the MAPK/ERK pathway[J]. J Cell Physiol, 2001, 187(1):73-80.
[4] Shefer G, Partridge TA, Heslop L, et al. Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells[J]. J Cell Sci, 2002, 115(Pt 7):1461-1469.
[5] Alexsandra da Silva Neto Trajano L, da Silva CL, de Carvalho SN, et al. Cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser[J]. Lasers Med Sci, 2016, 31(5):841-848.
[6] Wang X, Huang N, Yang M, et al. FTO is required for myogenesis by positively regulating mTOR-PGC-1α pathway-mediated mitochondria biogenesis[J]. Cell Death Dis, 2017, 8(3):e2702.
[7] Cunningham JT, Rodgers JT, Arlow DH, et al. mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex[J]. Nature, 2007, 450(7170):736-740.
[8] Martins F, Renno AC, Oliveira F, et al. Low-level laser therapy modulates musculoskeletal loss in a skin burn model in rats[J]. Acta Cir Bras, 2015, 30(2):94-99.
[9] Rudnicki MA, Schnegelsberg PN, Stead RH, et al. MyoD or Myf-5 is required for the formation of skeletal muscle[J]. Cell, 1993, 75(7):1351-1359.
[10] Mcintosh LM, Garrett KL, Megeney L, et al. Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin- and MyoD-deficient muscles[J]. Anat Rec, 1998, 252(2):311-324.
[11] Alves AN, Ribeiro BG, Fernandes KP, et al. Comparative effects of low-level laser therapy pre-and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair[J]. Lasers Med Sci, 2016, 31(4):679-685.
[12] Brunelli RM, Rodrigues NC, Ribeiro DA, et al. The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion[J]. Lasers Med Sci, 2014, 29(1):91-96.
[13] Macedo AB, Moraes LH, Mizobuti DS, et al. Low-level laser therapy (LLLT) in dystrophin-deficient muscle cells:Effects on regeneration capacity, inflammation response and oxidative stress[J]. PLoS One, 2015, 10(6):e0128567.
[14] Houreld NN, Masha RT, Abrahamse H. Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells[J]. Laser Surg Med, 2012, 44(5):429-434.
[15] Hay N, Sonenberg N. Upstream and downstream of mTOR[J]. Genes Dev, 2004, 18(16):1926-1945.
[16] Martin DE, Hall MN. The expanding TOR signaling network[J]. Curr Opin Cell Biol, 2005, 17(2):158-166.
[17] Sperandio FF, Giudice FS, Correa L, et al. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway[J]. J Biophotonics, 2013, 6(10):839-847.

Effects of laser stimulation with different power on oxygen consumption rate of C₂C₁₂ myoblasts in mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments