支链氨基酸对模拟失重大鼠肌萎缩的干预作用

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摘要目的观察支链氨基酸(BCAA)对模拟失重条件下大鼠肌萎缩的干预效果。方法尾部悬吊法建立模拟失重模型，SD雄性大鼠随机分为地面对照组(GC组)、尾吊对照组(TC组)及尾吊支链氨基酸干预组(TBI组)，GC组和TBI组经人工灌胃的方式分别给予相应剂量的纯净水和支链氨基酸溶液，饲养4周后取血和比目鱼肌(SOL)，测定其相关各项指标。结果与GC组比较，TC组大鼠比目鱼肌湿重和湿重体质量比均显著减少(P<0.01)，血清BUN、Ub水平显著升高(P<0.05)，IGF-1、TESTO、SOD、GSH-Px水平显著降低(P<0.05)，Atrogin-1蛋白表达水平增加32%。与TC组比较，TBI组大鼠SOL的湿重和湿重体质量比均显著增加(P<0.01);血清IGF-1、TESTO、Ub均增加，其中TESTO水平增加显著(P<0.05)，BUN水平减小，血清SOD、GSH-Px活力均显著增加(P<0.05);Atrogin-1蛋白表达水平显著减小(P<0.05)。结论 BCAA可能通过刺激体内的合成代谢激素的分泌、提高机体抗氧化能力以及抑制Atrogin-1蛋白表达等机制，在一定程度上促进骨骼肌蛋白的合成，抑制骨骼肌蛋白的分解，从而减轻尾吊条件导致的肌萎缩。

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关键词 ：支链氨基酸, 模拟失重, 肌萎缩, 干预作用

Abstract：Objective To investigate the protective effect of branched-chain amino acid(BCAA)on muscle atrophy in weightlessness-simulated rats.Methods Weightlessness was simulated by a tail-suspended model in rats. SD rats were randomly divided into 3 groups: ground control group (GC), tail-suspended control group (TC) and tail-suspended with BCAA intervention group (TBI).All the rats were successively fed with normal diets. The rats in GC and TC groups received pure water, and those in TBI group were given pure water and intervened with BCAA solution via intragastric administration respectively. The biochemical indices of serum and soleus (SOL) were detected after 4 weeks.Results Compared with GC group, the muscle wet weight(MWW) of and the ratio of MWW to body weight of rats in the TC group were significantly decreased(P<0.01),the level of serum BUN and Ub was significantly increased(P<0.05),the level of serum IGF-1、TESTO、SOD、 and GSH-Px was significantly decreased(P<0.05), and the expression of atrogin-1 protein in SOL decreased by 32%. Compared with TC group, the muscle wet weight(MWW) and the ratio of MWW to body weight were significantly increased (P<0.01) in TBI group,so was the serum level of IGF-1、TESTO、Ub, SOD and GSH-Px,,but the serum BUN and the expression of atrogin-1 protein in SOL were decreased,while the level of SOD and GSH-Px was increased significantly(P<0.05). (P<0.05).Conclusion BCAA may promote the synthesis of SOL protein, but inhibit its breakdown to some extent,alleviating SOL atrophy in tail-suspended rats. The mechanism potentially lies in facilitating the secretion of anabolic hormone and antioxidation ability,and inhibiting the expression of atrogin-1 protein.

Key words： BCAA weightlessness-simulated muscle atrophy protective effect

出版日期: 2016-09-14

基金资助:中国载人航天工程基金资助项目(No.92110HY-4)国家自然科学基金资助项目(No.81202612)

作者简介: 李红毅(1979-)，男，研究生，航天营养学硕士。从事航天营养基础研究工作。

引用本文:

李红毅，白树民，黄贱英，韩晓龙，朱德兵，雷浪伟，陈朴. 支链氨基酸对模拟失重大鼠肌萎缩的干预作用[J]. 解放军预防医学杂志, 2016, 34(4): 468-470.
LI Hongyi,BAI Shumin,HUANG Jianying, HAN Xiaolong, ZHU Debing,LEI Langwei,CHEN Pu. Protective Effect of Branched-Chain Amino Acid on Muscle Atrophy in Weightlessness-Simulated Rats. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(4): 468-470.

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http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/ 或 http://manu37.magtech.com.cn/Jwk_jsyxkx/jfj/CN/Y2016/V34/I4/468

(1) 韩忠宇,贾懿劼,田京.失重造成肌萎缩的研究与进展(J).中国组织工程研究,2013,17(28):5249.
(2) 吴瑞,余小燕.补充支链氨基酸在运动训练中的研究与进展(J).西安文理学院学报,2010,13(2):13.
(3) FERRANDO A A,PADDON JONE,WOLFE R R.Alterations in protein metabolism during space flight and inactivity(J).Nutrition,2002,18(10):837.
(4) 陈杰,马进,丁兆平,等.一种模拟长期失重影响的大鼠尾部悬吊模型(J).空间科学学报,1993,13:159.
(5) DAY C S. Insulin growth factor-1 decreases muscle atrophy following denervation(J).Microsurgery,2002,22:144.
(6) KAMEL H K,MAAS D,DUTHIE E H J.Role of hormones in the pathogenesis and management of sarcopenia(J).Drugs and Aging,2002,19:865.
(7) CAO P R, KIM H J, LECKER S H. Ubiquitin-protein ligases in muscle wasting(J).Int J Biochem Cell Biol,2005,37: 25.
(8) LAWLER J M, SONG W, DEMAREE S R. Hindlimb unloading increases oxidative stress and disrupts antioxidant capacity in skeletal muscle(J).Free Radic Biol Med,2003,35:9.