Abstract：Objective To evaluate the effect of quercetin on axonal growth and functional recovery in mice after spinal cord injury.Methods Twenty-four C57BL/6 female mice were randomly and evenly divided into two groups. Complete compression injury at the spinal cords of T10 was induced in both groups . The mice in therapeutic group were administered with quercetin (50 mg/kg) while control group received the same dose of saline by intraperitoneal injection at 30min before injury, and immediately after injury, and then administered once a day from 1 to 10 days post-injury. The mice in control group received the same dose of saline with an equally long duration. Biotinylated dextran amine (BDA) was injected into the sensorimotor cortex at 14 days post-injury to trace the corticospinal tract (CST). Counterstaining was performed with glial fibrillary acidic protein (GFAP) to label the lesion boundary. The CST retraction and pullulation were observed under a fluorescent microscope. BMS was performed to assess motor functional recovery at 1, 3, 7, 14 and 28 days post-injury.Results Compared with control group, the ratio of total labeled sprouts/axons was significantly increased, the ratio of total labeled retraction bulbs/axons decreased,and the distance of the bundle to lesion border decreased after quercetin treatment at 28 days post-injury. The BMS scores were also significantly improved in therapeutic group (P<0.01).Conclusion Quercetin can promote axonal pullualtion, prevent axonal retraction and improve motor functional recovery after spinal cord injury in mice.
党圆圆，张洪钿，杨艺，籍新潮，张岩，刘宁，徐如祥. 槲皮素促进小鼠脊髓损伤后轴突生长及功能恢复研究[J]. 解放军预防医学杂志, 2016, 34(4): 471-474.
DANG Yuanyuan, ZHANG Hongdian, YANG Yi, JI Xinchao, ZHANG Yan, LIU Ning, XU Ruxiang. Quercetin Promotes Axonal Growth and Functional Recovery after Spinal Cord Injury in Mice. Journal of Preventive Medicine of Chinese People's Liberation Army, 2016, 34(4): 471-474.
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