目的 探究以燕麦β-葡聚糖为基础的不同益生元组合配方对肠道健康的影响。方法 采用人体粪便体外发酵方法,对两个以燕麦β-葡聚糖为基础的益生元组合配方进行24 h和48 h发酵,通过qPCR和16S rDNA的方法探究配方对肠道菌群的影响。之后采用双中心平行双盲随机对照人群试验,选取192名排便困难的18~65岁的健康成人,随机分为3组,分别干预28 d,试验期间收集受试者的粪便,并记录受试者排便情况。采用线性混合效应模型、t检验和协方差分析探讨益生元组合配方对肠道菌群、排便状况和肠道健康状况的影响。结果 体外试验和人群试验显示,相比于对照组,以燕麦β-葡聚糖为基础的益生元组合配方可以促进肠道有益菌普拉梭菌（Faecalibacterium prausnitzii）、黏蛋白艾克曼氏菌（Akkermansia muciniphila）、霍尔德曼氏菌属（Holdemania）和布劳特氏菌属（Blautia）的增殖（P<0.05）。经人群试验验证,相比于普通膳食纤维,燕麦β-葡聚糖为基础的两个益生元组合能改善排便,以及腹胀、消化不良等肠道健康问题（P<0.05）。结论 以燕麦β-葡聚糖为基础的益生元组合配方,可促进肠道有益菌增殖,改善肠道健康。
Abstract
Objective To investigate the effects of oat β-glucan based prebiotic blending formulas on gut health. Methods The 24 h and 48 h in vitro fermentations on two oat β-glucan based prebiotic blending formulas using human feces were conducted. The effects of two formulas on gut bacteria were explored by qPCR and 16S rDNA approaches. Subsequently, a two-center parallel double-blind randomized controlled human trial was conducted in 192 adults with defecation difficulty. The participants were randomly assigned to one of three groups with a 28-day intervention. During the intervention, human fecal samples were collected and defecation feelings were investigated. The data were analyzed using generalized mixed linear model, t-test and ANCOVA. Results The results of both in vitro test and human trial found that, compared to control group, oat β-glucan based prebiotic blending formulas could promote the growth of Faecalibacterium prausnitzii, Akkermansia muciniphila, Holdemania and Blautia (P<0.05). Oat β-glucan based prebiotic blending formulas also improved the defecation difficulty and digestive feeling(P<0.05). Conclusion Oat β-glucan based prebiotic blending formulas can promote the growth of gut beneficial bacteria, and improve gut health in adults with defecation difficulty.
关键词
燕麦β-葡聚糖 /
益生元 /
体外发酵 /
人群试验 /
肠道菌群 /
排便
Key words
oats β-glucan /
prebiotic /
invitro fermentation /
human trial /
gut bacteria /
defecation
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