目的 探究马齿苋多糖(Portulaca oleracea polysaccharide, POP)对老龄大鼠肠道菌群结构的影响。方法 20只SD老龄大鼠(21月龄)随机分为2组,每组10只。对照组(Con)大鼠饲喂实验鼠维持颗粒料,干预组(POP)大鼠饲喂添加1.0% POP维持颗粒料,自由摄食和饮水,持续15 w。实验结束后,采用16S rRNA高通量测序技术分析肠道菌群结构。结果 两组共有6087个相同的操作分类单元(OTUs),Con组有1025个特征OTUs,POP组有1131个特征OTUs。两组动物肠道菌群有相似的α多样性,组间β多样性有显著性差异。摄入POP后,老龄大鼠肠道菌群中的梭杆菌门(Fusobacteria)、乳杆菌属(Lactobacillus)、瘤胃梭菌属(Ruminiclostridium_9)、Intestinimonas菌属、粪杆菌属(Faecalibacterium)、脱硫弧菌属(Desulfovibrio)、嗜果胶拟杆菌属([Bacteroides]_pectinophilus_group)和瘤胃梭菌(Ruminiclostridium)相对丰度显著降低,而副拟杆菌属(Parabacteroides)、瘤胃菌科(Ruminococcaceae_UCG-005)和Marvinbryantia菌属相对丰度显著增加。老龄大鼠摄入POP后可能上调细胞过程、新陈代谢和生物系统相关基因的表达,下调环境信息处理、人类疾病和遗传信息处理等相关基因的表达。结论 POP可影响老龄大鼠肠道菌群结构,瘤胃菌科(Ruminococcaceae_UCG.005)和副拟杆菌属(Parabacteroides)是主要的生物标志物。
Abstract
Objective To investigate the effects of Portulaca oleracea polysaccharides (POP) on intestinal flora structure in aged rats. Methods Twenty elderly SD rats (21 months of age) were randomly divided into two groups with ten rats in each group. Control group (Con) rats were fed a normal diet, and intervention group (POP) rats were fed a diet supplemented with 1.0% POPs. All rats were fed for 15 consecutive weeks. At the end of the experiment, high-throughput sequencing technology (16S rRNA) was used to analyze the composition of intestinal flora. Results The results showed that there were 6087 identical operational taxonomic units (OTUs) in the two groups. Con group had 1025 characteristic OTUs, and POP group had 1131 characteristic OTUs. The intestinal flora of the two groups had similar alpha-diversity, but beta-diversity was significantly different between two groups. After ingestion of Portulaca oleracea polysaccharides, the relative abundances of Fusobacteria, Lactobacillus, Ruminiclostridium_9, Intestiniamonas, Faecalibacium, Desulfovibrio, [Bacteroides]_pectinophilus_group and Ruminiclostridium in the intestinal flora of aged rats decreased significantly, while the relative abundances of Parabacteroides, Ruminococcaceae_UCG-005 and Marvinbryantia increased significantly. The ingestion of Portulaca oleracea polysaccharides up-regulated the expression of genes related to cellular processes, metabolism and organismal systems, and down-regulated the expression of genes related to environmental information processing, human disease and genetic information processing in aged rats. Conclusion POP influences the structure of intestinal microflora in aged rats, and the Ruminococcaceae_UCG.005 and Parabacteroides are the main biomarkers.
关键词
马齿苋多糖 /
肠道菌群 /
老龄大鼠
Key words
Portulaca oleracea L. polysaccharides /
intestinal flora /
aged rat
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基金
国家自然科学基金（No. 31860711）; 江西省教育厅科技项目重点项目（No. GJJ211006）
