目的 探讨高蛋白膳食对限食大鼠尿液代谢组的影响。方法 Wistar雄性大鼠,按体重随机分三组（每组8只）：正常对照组,喂15%酪蛋白合成饲料,自由摄食;限食组按正常对照组摄食量的30%对喂15%酪蛋白合成饲料;补蛋白组按正常对照组摄食量30%对喂30%酪蛋白合成饲料。实验2 w后收集大鼠24 h的尿液,以核磁共振谱仪检测尿液中代谢物的变化情况。结果 限食和补充蛋白两种干预措施均显著改变了尿液中代谢产物谱。限食使大鼠尿液中的柠檬酸、α-酮戊二酸、N-乙酰糖蛋白水平降低,牛磺酸水平升高;而补蛋白组柠檬酸、α-酮戊二酸、N-乙酰糖蛋白水平又高于相应的限食组,牛磺酸水平也高于相应的限食组。结论 高蛋白膳食具有改善限食大鼠体内部分物质代谢紊乱的作用。
Abstract
Objective To investigate the effects of high-protein diet on urinary metabolome in rats with food restriction. Methods Twenty-four male Wistar rats were randomly divided into three groups (8 per group). The normal control group received 15% casein diet and water ad-libitum. The food restriction group was pair-fed the 15% casein diet with 30% food intake of the normal control. The protein supplemented group was pair-fed 30% casein diet with the 30% food intake of the normal control. Urine samples were collected 2 weeks after pair feeding. Nuclear magnetic resonance spectrometer was used to investigate the changes of urinary metabolite profiles. Results Both food restriction and protein supplemented groups significantly altered the urinary metabolites. Food restriction led to a decrease in the levels of citric acid, α-ketoglutaric acid, N-acetyl glycoprotein and an increase in the level of taurine in the urine, while the levels of citric acid, α-ketoglutaric acid, and N-acetyl glycoprotein were higher in the protein supplemented group than in the food restriction group, and the level of taurine was also higher than in the food restriction group. Conclusion A high-protein diet is helpful in improving metabolic disorders in the food restricted rats.
关键词
高蛋白膳食 /
限食 /
核磁共振 /
尿液代谢组 /
大鼠
Key words
high protein diet /
food restriction /
nuclear magnetic resonance /
urinary metabolites /
rats
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基金
国家重点研发计划（No. 2023YFF1105200）
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