限时喂养对持续光照下小鼠棕色脂肪组织糖脂代谢调控的影响

胡雨雨; 张冠宇; 武帅; 李曦; 李军; 杨丹凤

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营养学报 ›› 2026, Vol. 48 ›› Issue (1) : 82-90.

论著

限时喂养对持续光照下小鼠棕色脂肪组织糖脂代谢调控的影响

胡雨雨^1,2, 张冠宇², 武帅², 李曦², 李军¹, 杨丹凤²

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EFFECTS OF TIME- RESTRICTED FEEDING ON GLYCOLIPID METABOLISM OF BROWN ADIPOSE TISSUE IN MICE UNDER CONTINUOUS LIGHT EXPOSURE

HU Yu-yu^1,2, ZHANG Guan-yu², WU Shuai², LI Xi², LI Jun¹, YANG Dan-feng²

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摘要

目的探究限时喂养（time-restricted feeding, TRF）对持续光照下小鼠棕色脂肪组织(brown adipose tissue, BAT)糖脂代谢调控的影响。方法将132只小鼠随机分为正常光照（LD）组、持续光照（LL）组与持续光照下限时喂养（LL+TRF）组,各组按取材时间再分为4个亚组（n=11）;实验2 w后于次日早上8:00开始每隔6h收集一批小鼠的血清与BAT;实时荧光定量PCR检测LD组、LL组、LL+TRF组小鼠BAT中时钟基因mRNA在24 h内的水平;通过余弦拟合分析持续光照对小鼠BAT昼夜节律的干扰及TRF的干预效果;采用相关方法检测小鼠BAT中糖脂代谢相关基因、血清葡萄糖与甘油三酯在24 h内的变化水平,并采用Western Blot检测BAT中GLUT1与GLUT4蛋白表达量。结果与LD组相比,LL组小鼠摄食节律紊乱、摄食量减少,BAT中多个核心时钟基因昼夜节律消失,糖脂代谢相关基因表达受抑,血糖血脂水平紊乱;与LL组相比,LL+TRF组小鼠BAT在日间出现Glut1表达下调和血糖升高,夜间出现Lpl表达下调和血脂升高,且BAT内参与脂肪酸从头合成的关键基因表达显著上调。结论在持续光照环境下,TRF能通过调节BAT的糖脂代谢相关基因表达,对血脂和血糖水平具有一定影响。

Abstract

Objective To explore the effects of time-restricted feeding (TRF) on glycolipid metabolism in brown adipose tissue (BAT) of mice under continuous light exposure. Methods A total of 132 mice were randomly divided into three groups: a normal light (LD) group, a continuous light (LL) group, and a continuous light plus time-restricted feeding (LL+TRF) group. Each group was further divided into 4 subgroups according to sampling time points (n=11). After 2 weeks of intervention, serum and BAT samples were collected every 6 h starting at 8:00 a.m. on the following day. qPCR was used to detect the mRNA levels of clock genes in BAT within 24 h. Cosine fitting analysis was performed to evaluate the disruption of circadian rhythms in BAT by continuous light and the intervention effect of TRF. Meanwhile, the 24 h expression changes of glycolipid metabolism-related genes in BAT, as well as serum glucose and triglyceride levels, were measured. The protein expression levels of GLUT1 and GLUT4 in BAT were detected by Western blot. Results Compared with the LD group, mice in the LL group showed disrupted feeding rhythms and decreased food intake. The circadian rhythms of multiple core clock genes in BAT were abolished, the expression of glycolipid metabolism-related genes was inhibited, and serum glucose and lipid levels were dysregulated. Compared with the LL group, mice in the LL+TRF group showed downregulated Glut1 expression in BAT and elevated blood glucose levels during the light phase, as well as downregulated Lpl expression in BAT and elevated blood lipid levels during the dark phase. Meanwhile, the expression of key genes involved in de novo lipogenesis in BAT was significantly upregulated in this group. Conclusion Under continuous light exposure, TRF can affect serum glucose and lipid levels by regulating the expression of glycolipid metabolism-related genes in BAT.

导出引用

胡雨雨, 张冠宇, 武帅, 李曦, 李军, 杨丹凤. 限时喂养对持续光照下小鼠棕色脂肪组织糖脂代谢调控的影响[J]. 营养学报. 2026, 48(1): 82-90

HU Yu-yu, ZHANG Guan-yu, WU Shuai, LI Xi, LI Jun, YANG Dan-feng. EFFECTS OF TIME- RESTRICTED FEEDING ON GLYCOLIPID METABOLISM OF BROWN ADIPOSE TISSUE IN MICE UNDER CONTINUOUS LIGHT EXPOSURE[J]. Acta Nutrimenta Sinica. 2026, 48(1): 82-90

中图分类号： Q493.4 Q95-33

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