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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (2): 116-120.doi: 10.13459/j.cnki.cjap.2016.02.006

• 研究论文 • 上一篇    下一篇

NADPH氧化酶抑制剂apocynin对力竭运动大鼠运动性蛋白尿的影响

陈丽娜, 周刚, 吴乐, 侯少华   

  1. 湖南大学体育学院, 长沙 410082
  • 收稿日期:2015-05-25 修回日期:2015-11-11 出版日期:2016-03-28 发布日期:2018-06-12
  • 通讯作者: 周刚,Tel:13548644844;E-mail:zg460@126.com E-mail:zg460@126.com
  • 基金资助:
    湖南省自然科学基金资助(12JJ3093)

Effect of NADPH oxidase inhibitor apocynin on the exercise induced proteinuria in rats

CHEN Li-na, ZHOU Gang, WU le, HOU Shao-hua   

  1. College of Sport, Hunan University, Changsha 410082, China
  • Received:2015-05-25 Revised:2015-11-11 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    湖南省自然科学基金资助(12JJ3093)

摘要: 目的:研究NADPH氧化酶抑制剂apocynin对力竭运动大鼠运动性蛋白尿产生的影响及其机制。方法:32只SD雄性大鼠随机分为安静对照组(C组)、对照+药物组(CA组)、力竭运动组(E组)、力竭运动+药物组(EA组)。药物注射按10 mg/kg体重,每天一次,连续3 d,并在末次药物注射1 h后进行一次性跑台力竭运动。测定运动后尿UP、血液BUN水平、肾脏ROS浓度、NOS活性、NOS与3-NT蛋白含量。结果:结果显示,E组UP、肾脏ROS、iNOS含量及活性、3-NT明显升高,而EA组的这些指标与C组相比无显著性差异。结论:力竭运动可明显增加肾组织NADPH氧化酶活性,从而产生大量的ROS,后者可迅速地与由肾脏iNOS催化生成的NO反应,产生过量的ONOO-,诱发运动性蛋白尿的生成。

关键词: NADPH氧化酶, 运动性蛋白尿, 活性氧, 过氧亚硝基阴离子, 一氧化氮

Abstract: Objective: To investigate the effect of NADPH oxidase inhibitor apocynin on exercise-induced proteinuria and its related mechanism. Methods: Thirty-two SD rats were randomly divided into the control group (group C), control + drug group (group CA), exhaustive exercise group (group E), exhaustive exercise + drug group (group EA). The rats were administrated apocynin at 10 mg/kg weight,once a day for three days, and one hour after the drug injection, a one-time exhaustive exercise was performed. After exhaustive exercise, urine protein, blood urea nitrogen (BUN), and kidney reactive oxygen species (ROS) concentration, NOS activity, NOS and 3-NT concentration were detected. Results: In comparison to control group urinary protein (UP), ROS, inductible nitric oxide synthase (iNOS), 3-NT levels increased significantly in group E while those in group EA did not change. Conclusion: The elevated renal NADPH oxidase activity by exhaustive exercise induced ROS that can rapidly react with NO, and then produces excess peroxynitrite, which contributes to occurrence of exercise-induced proteinuria.

Key words: NADPH oxidase, exercise-induced proteinuria, reactive oxygen species (ROS), peroxynitrite (ONOO-), nitric oxide

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