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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (3): 274-277.doi: 10.13459/j.cnki.cjap.2016.03.022

• 研究论文 • 上一篇    下一篇

激活多巴胺I类受体对氧化性低密度脂蛋白诱导的人单核细胞THP-1分泌NO/NOS的影响

柳磊, 时飒, 李鸿珠, 李弘, 徐长庆   

  1. 哈尔滨医科大学基础医学院病理生理学教研室, 黑龙江 哈尔滨 150086
  • 收稿日期:2015-03-09 修回日期:2015-10-20 出版日期:2016-05-28 发布日期:2018-06-12
  • 通讯作者: 李弘,Tel:0451-86674548;E-mail:drlihong1971@163.com E-mail:drlihong1971@163.com
  • 基金资助:
    黑龙江省自然科学基金项目资助(D200880);黑龙江省教育厅课题(12521181)

Effects of activation of dopamine type I receptor on the produc-tion of NO/NOS in ox-LDL activated THP-1 cells

LIU Lei, SHI Sa, LI Hong-zhu, LI Hong, XU Chang-qing   

  1. Department of Pathophysiology, Harbin Medical University, Harbin 150086, China
  • Received:2015-03-09 Revised:2015-10-20 Online:2016-05-28 Published:2018-06-12
  • Supported by:
    黑龙江省自然科学基金项目资助(D200880);黑龙江省教育厅课题(12521181)

摘要: 目的:探讨激活多巴胺Ⅰ类受体(DR1)对氧化型低密度脂蛋白(ox-LDL)诱导的人单核细胞(THP-1)分泌一氧化氮/一氧化氮合酶(NO/NOS)的影响及可能机制。方法:THP-1细胞经佛波酯PMA诱导分化,分为正常对照组(control),氧化型低密度脂蛋白处理组(ox-LDL),DR1激动剂干预组(SKF),DR1阻断剂干预组(SCH),ERK阻断剂干预组(PD98059);应用油红O染色法鉴定泡沫细胞;硝酸还原法检测NO、NOS的变化情况;免疫荧光和Western blot检测各组细胞蛋白表达情况。结果:ox-LDL刺激48 h可形成泡沫细胞;DR1在THP1细胞上表达,ox-LDL刺激后,DR1蛋白表达降低(P<0.01);激活DR1受体能够明显抑制由ox-LDL引起的NO、iNOS增多(P<0.01);在MAPK阻断剂PD98059存在的情况下,SKF的作用部分丧失。结论:激活DR1受体可抑制ox-LDL引起的THP-1细胞NO的大量产生,此过程可能由ERK信号通路所介导。

关键词: 多巴胺受体, 巨噬细胞, 一氧化氮, 单核细胞, 低密度脂蛋白

Abstract: Objective:To study the effect of excited dopamine type I receptor on the production of nitric oxide/nitric oxide synthase(NO/NOS)in ox-LDL activated THP-1 cells and the possible mechanism. Methods:Cultured THP-1 cells activated by PMA were randomly assigned in the following groups:control group (control), oxidized low density lipoprotein group (ox-LDL), dopamine receptor 1(DR1) agonist group (SKF), DR1 antagonist group (SCH), ERK blocker group (PD98059). Oil Red O staining was used to identify the accumulation of cellular lipid. The levels of NO and NOS in the supernatant of THP-1 were assayed by nitrate reductase method. The protein expression of DR1, p-ERK and ERK were obtained by Western blot and immunity fluorescence. Results:After 48 h of incubation of ox-LDL, accumulation of lipid in the cytoplasm was found in most THP-1 cells. Compared with control group, DR1 protein expression was reduced in ox-LDL-induced cells(P<0.01). Activation of DR1 agonist decrease the production of NO and iNOS(P<0.01), and PD98059 partly reversed the above effect. Conclusion:Activation of DR1 can inhibit the production of NO/NOS in ox-LDL-induced THP-1 cells, which may be related with ERK pathway.

Key words: dopamine receptor(DR), foam cells, nitric oxide(NO), THP-1 cells, LDL

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