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中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (1): 51-55.doi: 10.12047/j.cjap.5448.2017.012

• 研究论文 • 上一篇    下一篇

十全育真汤对H22荷瘤小鼠免疫功能的影响

朱云超, 詹光杰, 袁德培, 杨付明, 黄琼   

  1. 湖北民族学院医学院, 恩施 445000
  • 收稿日期:2015-11-17 修回日期:2016-10-25 出版日期:2017-01-28 发布日期:2018-06-19
  • 通讯作者: 朱云超,Tel:13972407721,E-mail:zhongzhen4002@163.com E-mail:zhongzhen4002@163.com
  • 基金资助:
    国家中医药管理局公共卫生专项资金项目(财社[2010]91号);湖北民族学院博士科研启动基金项目(MY2010B004)

Effects of Shiquanyuzhentang on immunologic function of H22 tumor-bearing mouse

ZHU Yun-chao, ZHAN Guang-jie, YUAN De-pei, YANG Fu-ming, HUANG Qiong   

  1. School of Medical Science, HuBei University for Nationalities, Enshi 445000, China
  • Received:2015-11-17 Revised:2016-10-25 Online:2017-01-28 Published:2018-06-19
  • Supported by:
    国家中医药管理局公共卫生专项资金项目(财社[2010]91号);湖北民族学院博士科研启动基金项目(MY2010B004)

摘要: 目的:研究十全育真汤对荷瘤小鼠免疫功能的影响,探讨十全育真汤抗肿瘤的作用机制。方法:SPF级雄性昆明种小鼠30只,制成荷H22小鼠肝癌细胞移植瘤模型,随机分为模型组、阳性对照组及十全育真汤组(n=10);另选择未接种小鼠10只为正常对照组。正常对照组、模型组每天按10 ml/kg灌胃生理盐水及蒸馏水,阳性对照组、十全育真汤组每天按8 g/kg、18 g/kg灌胃参一药液(80 mg/ml)与十全育真汤剂。连续给药14 d后处死小鼠,测量胸腺、脾脏指数及抑瘤率,检测外周血中白细胞、淋巴细胞含量及T细胞亚群CD3、CD4、CD8细胞百分比,血清中白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)及干扰素-β(IFN-β)的含量,淋巴细胞增殖能力及NK细胞杀伤功能。结果:较正常对照组,十全育真汤组小鼠体重明显增加,脾脏指数显著增大(P<0.05);白细胞、淋巴细胞CD4、CD8、CD3及TNF-α含量明显升高(P<0.05);IL-2、IFN-β含量显著下降(P<0.05);淋巴细胞增殖能力、NK细胞杀伤功能明显升高(P<0.05)。与模型组相比,十全育真汤组小鼠胸腺、脾脏指数显著增大(P<0.05);白细胞、淋巴细胞CD3、CD4、IL-2、IFN-β及TNF-α含量明显升高(P<0.05),CD8含量则显著降低(P<0.05);NK细胞杀伤功能及淋巴细胞增殖能力显著增加(P<0.05)。结论:十全育真汤可促进H22荷瘤小鼠免疫器官的生长,增强机体免疫功能,有利于肿瘤机体的恢复。

关键词: 十全育真汤, H22荷瘤小鼠, 免疫

Abstract: Objective: To investigate the effects of Shiquanyuzhentang (Traditional Chinese Medicine) on the immunologic function of tu-mor-bearing mice and its mechanism of antitumor.Methods: Thirty SPF grade male Kunming mice transplanted with H22 hepatocellular carci-noma were divided into three groups randomly:model group,positive control group and Shiquanyuzhentang group(n=10). Another 10 mice were selected as normal control group. Normal control group and model group received normal saline and distilled water supplementation by 10 mL/kg everyday. Positive control group and Shiquanyuzhentang group received Shengyi(80 mg/ml)and Shiquanyuzhentang decoction at the doses of 8 g/kg and 18 g/kg respectively everyday. After 14 days of continuous administration, the mice were killed and the thymus, spleen index, tumor inhibition rate,peripheral blood leukocytes,lymphocyte content,cell percentage of T cell subsets CD3, CD4, CD8,interleukin 2 (IL-2)in serum,tumor necrosis factor-α(TNF-α),interferon β(IFN-β) content,lymphocyte proliferation ability and NK cell were measured.Results: Compared with normal control group, the weight of mice and thymus and spleen index of the Shiquanyuzhentang group were increased significantly(P<0.05);leukocyte and lymphocyte CD3、CD4、CD8 and TNF-α were increased greatly(P<0. 05),IL-2 and IFN-β were de-creased significantly(P<0.05). Compared with model group,thymus and spleen index of Shiquanyuzhentang group were increased significant-ly(P<0.05);leukocyte and lymphocyte CD3、CD4、IL-2、IFN-β and TNF-α of Shiquanyuzhentang group were increased significantly(P<0. 05),CD8 content was decreased significantly(P<0.05);NK cell and lymphocyte proliferation were increased significantly(P<0.05).Conclusion: Shiquanyuzhentang can promote the growth of immune organs in mice bearing H22, enhance immune function and is beneficial to the recovery of tumor body.

Key words: Shiquanyuzhentang, H22 tumor-bearing mouse, immunity

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