海洛因诱导大鼠穿梭觅药潜伏期边缘前区θ振荡的改变

doi:10.13459/j.cnki.cjap.2016.05.012

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (5): 431-437.doi: 10.13459/j.cnki.cjap.2016.05.012

海洛因诱导大鼠穿梭觅药潜伏期边缘前区θ振荡的改变

叶政¹, 虞冉¹, 李晶¹, 李敏¹, 朱再满¹, 王丹丹², 潘群皖¹

1. 皖南医学院生理教研室, 安徽芜湖 241000;
2. 临床医学院, 安徽芜湖 241000

收稿日期:2015-09-28 修回日期:2016-05-15 出版日期:2016-09-28 发布日期:2018-06-20
通讯作者: 潘群皖,Tel:0553-3832473;E-mail:panqunw@163.com E-mail:panqunw@163.com
基金资助:
安徽省高校自然科学研究重点项目（KJ2015A193）；安徽省高校自然科学研究重点项目（KJ2016A720）

Changes of theta oscillation of prelimbic cortex in heroin-induced CPP rats

YE Zheng¹, YU Ran¹, LI Jing¹, LI Min¹, ZHU Zai-man¹, WANG Dan-dan², PAN Qun-wan¹

1. Department of Physiology, Wangnan Medical College, Wuhu 241001, China;
2. Clinical Medical College, Wangnan Medical College, Wuhu 241001, China

Received:2015-09-28 Revised:2016-05-15 Online:2016-09-28 Published:2018-06-20
Supported by:
安徽省高校自然科学研究重点项目（KJ2015A193）；安徽省高校自然科学研究重点项目（KJ2016A720）

摘要/Abstract

摘要： 目的：遥测海洛因诱导条件性位置偏爱大鼠穿梭觅药潜伏期边缘前区（PrL）的局部场电位（LFPs），探索与觅药动机形成相关的神经振荡改变。方法：将PrL区埋藏电极的大鼠随机分为手术对照组和海洛因诱导CPP组。利用视频监视和脑电无线遥测技术，记录大鼠黑-白箱穿梭觅药过程中的实时LFPs，通过快速傅立叶变化和小波包提取技术对原始LFPs进行分析。结果：与手术对照组比较，海洛因诱导CPP组大鼠黑-白箱穿梭潜伏期左右侧θ节律百分比增加，左侧γ3节律百分比增加，出现θ~γ3相位振幅耦合增强。MK-801微量注射PrL区后，大鼠觅药行为明显减少，左右侧θ节律百分比降低，左侧θ~γ3相位振幅耦合减弱。结论：本研究提示海洛因诱导CPP组大鼠PrL区θ振荡活动增强，左侧θ~γ3相位振幅耦合增强，可能与大鼠海洛因觅药动机和行为发动密切相关，这种电活动的改变也与PrL区谷氨酸能神经元及其受体的功能密不可分。

关键词: 海洛因, 条件性位置偏爱, 边缘前区, 局部场电位, θ振荡

Abstract: Objective: To investigate the relationship between the changes of neural oscillations and the drug-seeking motivation,record the telemetric local field potentials (LFPs) of the prelimbic cortex (PrL) in the latency of drug-seeking behavior of conditioned place preference (CPP) rats induced by heroin. Methods: The recording electrode was stereotactically implanted intothe PrL cortex of rats. The animals were then randomly divided into operation-only control and heroin-induced CPP groups, respectively. A CPP video system in combination with awireless telemetry device was used for recording LFPs when the rats shuttled between black-white chamber for drug-seeking. The LFPs were analyzed by fast Fourier transform (FFT) and wavelet packet extraction. Results: Compared with operation-only control group, the LFPs recorded in PrL area of heroin-induced CPP group of rat during black-white chamber shuttling showed that the percentage of θ rhythm were increased in right and left PrL, the percentage of γ3 rhythm was increased in left PrL, the phase-amplitude coupling of θ and γ3 was increased in left PrL. After MK-801 Maleate micro injection to PrL area,drug-seeking behavior of rat was markedly reduced and the percentage of θ oscillation was depressed,the phase-amplitude coupling of θ and γ3 was depressed in left PrL compared with operation-only control group. Conclusion: These results suggest that increase of θ oscillations of PrL area may be related to the drug-seeking motivation and behavior launching in heroin-induced CPP groups of rat. The changes of θ oscillations also have close relationship with glutamatergic neuron and the receptor of it on PrL area.

Key words: heroin, conditioned place preference, prelimbic cortex, local field potentials, theta oscillations

叶政, 虞冉, 李晶, 李敏, 朱再满, 王丹丹, 潘群皖. 海洛因诱导大鼠穿梭觅药潜伏期边缘前区θ振荡的改变[J]. 中国应用生理学杂志, 2016, 32(5): 431-437.

YE Zheng, YU Ran, LI Jing, LI Min, ZHU Zai-man, WANG Dan-dan, PAN Qun-wan. Changes of theta oscillation of prelimbic cortex in heroin-induced CPP rats[J]. CJAP, 2016, 32(5): 431-437.

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海洛因诱导大鼠穿梭觅药潜伏期边缘前区θ振荡的改变

Changes of theta oscillation of prelimbic cortex in heroin-induced CPP rats

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