高脂饲养青春期大鼠电击回避反应和海马CA3区场电位的改变

doi:10.13459/j.cnki.cjap.2016.06.013

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (6): 545-549.doi: 10.13459/j.cnki.cjap.2016.06.013

高脂饲养青春期大鼠电击回避反应和海马CA3区场电位的改变

王丹丹¹, 王羽¹, 汪镜静¹, 陈颖¹, 叶政², 李晶², 李敏², 朱再满², 潘群皖²

1. 皖南医学院临床医学院, 安徽芜湖 241000;
2. 皖南医学院生理教研室, 安徽芜湖 241000

出版日期:2016-11-28 发布日期:2018-06-19
通讯作者: 潘群皖,Tel:0553-3932473;E-mail:panqunw@163.com E-mail:panqunw@163.com
基金资助:
国家级大学生创新创业训练计划（201410368006）；安徽省高校自然科学基金重点项目（KJ2016A720）

The changes of electric shock avoidance response and local field potentials on hippocampal CA3 region of the adolescent rats with high fat feeding

WANG Dan-dan¹, WANG Yu¹, WANG Jing-jing¹, CHEN Ying¹, YE Zheng², LI Jing², LI Min², ZHU Zai-man², PAN Qun-wan²

1. Clinical Medical College, Wannan Medical College, Wuhu 241002, China;
2. Departenment of Physiology, Wannan Medical College, Wuhu 241002, China

Online:2016-11-28 Published:2018-06-19
Supported by:
国家级大学生创新创业训练计划（201410368006）；安徽省高校自然科学基金重点项目（KJ2016A720）

摘要/Abstract

摘要： 目的：观察高脂饲养至青春期的大鼠对电击回避反应和海马CA3区实时局部场电位变化。方法：断乳1周幼鼠改用基础饲料和高脂饲料分别喂养4周至青春期，分为基础饲料组（BF组）和高脂饲料组（HFD组），Y型迷宫电击回避训练方法，记录2组大鼠电击回避达学会标准的相关参数，同时无线遥测大鼠达标时海马CA3区实时局部场电位。结果：与BF组大鼠比较，HFD组大鼠体重明显增加，Y型迷宫电击回避训练1~2 d大鼠达标百分率、电击回避达标各项指标均略优于BF组；双侧海马CA3区局部场电位节律出现去同步化快波改变，右侧海马CA3区出现了θ波和γ 1波百分比的同步性增加，但无θ~γ 1波相位-振幅耦合出现。结论：幼年期短期高脂饮食至青春期的大鼠，尽管体重较基础饲料大鼠增加，但未见Y型迷宫电击回避反应能力和海马依赖性空间认知功能的减退。

关键词: 大鼠, 青春期大鼠, 电击回避训练, 海马CA3区, 局部场电位

Abstract: Objective: To investigate the changes of shock avoidance response in Y maze and the real-time local field potentials (LFPs) on hippocampal CA3 region in adolescent rats with high fat feeding. Methods: The juvenile rats of weaning 1 week were fed separately on basic feed (BF) and high fat diet (HFD) for 4 weeks until the puberty. Using the electric shock avoidance training method, the relevant parameters of reaching the learning standard of two groups of rats were recorded, and the LFPs of CA3 region in hippocampus were explored simultaneously by wireless telemetry. Results: Compared with the BF group, the body weight of HFD rats were increased significantly(P<0.05), the rats percentage of reaching the learning standard within 1 to 2 days and the various parameters of shock avoidance recorded in training were all superior to those of BF group rat,the LFP rhythm of bilateral hippocampal CA3 region appeared to the desynchronizd fast wave. The θ and γ1 rhythms of right hippocampal CA3 region shown synchronous increase, but there was no coupling formation in phase-amplitude of the θ and γ1 rhythms. Conclusion: From juvenile to adolescent with HFD intake, the adolescent rat gained an increase significantly in weight,but the ability of shock avoidance and the function of hippocampus-dependent spatial cognition in Y maze had not be expected decline.

Key words: rat, adolescent rat, electric shock avoidance training, hippocampal CA3 region, local field potentials

中图分类号:

王丹丹, 王羽, 汪镜静, 陈颖, 叶政, 李晶, 李敏, 朱再满, 潘群皖. 高脂饲养青春期大鼠电击回避反应和海马CA3区场电位的改变[J]. 中国应用生理学杂志, 2016, 32(6): 545-549.

WANG Dan-dan, WANG Yu, WANG Jing-jing, CHEN Ying, YE Zheng, LI Jing, LI Min, ZHU Zai-man, PAN Qun-wan. The changes of electric shock avoidance response and local field potentials on hippocampal CA3 region of the adolescent rats with high fat feeding[J]. CJAP, 2016, 32(6): 545-549.

参考文献

[1] Uranga RM, Bruce-Keller AJ, Morrison CD, et al. Intersec-tion between metabolic dysfunction, high fat diet consump-tion, and brain aging[J]. J Neurochem, 2010, 114(2):344-361.
[2] Kesby JP, Kim JJ, Scadeng M, et al. Spatial cognition in adult and aged mice exposed to high-fat diet[J]. PLoS One, 2015, 10(10):e0140034
[3] Boitard C, Etchamendy N, Sauvant J, et al. Juvenile, but not adult exposure to high-fat diet impairs relational memory and hippocampal neurogenesis in mice[J]. Hippocampus, 2012, 22(11):2095-2100.
[4] Boitard C, Cavaroc A, Sauvant J, et al. Impairment of hip-pocampal-dependent memory induced by juvenile high-fat diet intake is associated with enhanced hippocampal inflammation in rats[J]. Brain Behav Immun, 2014, 40:9-17.
[5] 钟志勇, 饶子亮, 谢超敏, 等. 不同饲料诱发大、小鼠肥胖的比较观察[J]. 实验动物与比较医学, 2011, 31(3):203-210.
[6] 汪炜炜, 王丹丹, 王丹, 等. 电击回避训练前后快回避反应大鼠海马CA3区神经振荡的变化[J]. 生理学报, 2015, 67(5):487-496.
[7] George P, Charles W. The rat brain in stereotaxic coordr-nates:6th ed[M]. Academic Press of Elsevier, Amsterdam, 2007:50.
[8] 王跃春, 王子栋, 孙黎明, 等. 动物学习记忆能力的Y 型迷宫测试法[J]. 暨南大学学报(自然科学版), 2001, 22(5):137-140.
[9] 白羽, 白家明, 李晶, 等. 吗啡诱导大鼠穿梭觅药状态下mPFC~PL区遥测脑电小波包提取及熵分析[J]. 生理学报, 2014, 66(6):675-682.
[10] Tort AB, Komorowski RW, Manns JR, et al. Theta-gamma coupling increases during he learningof item-context associa-tions[J]. Proc Natl Acad Sci U S A, 2009, 106(49):20942-20947.
[11] Canolty RT, Edwards E, Dalal SS, et al. High gamma power is phase-locked to theta oscillations in human neocortex[J]. Science, 2006, 313(5793):1626-1628.
[12] Ojeda SR. Urbanski HF:Puberty in the rat. In the physiolo-gy of reproduction[M]. New York, NY:RavenPress, 1994:363-409.
[13] Noble EE, Mavanji V, Little MR, et al. Exercise reduces diet-induced cognitive decline and increaseshippocampal brain-derived neurotrophic factor in CA3 neurons[J]. Neuro-biol Learn Mem, 2014, 114:40-50.
[14] Beilharz JE, Maniam J, Morris MJ. Short exposure to a diet rich in both fat and sugar or sugar alone impairs place, but not object recognitionmemory in rats[J]. Brain Behav Im-mun, 2014, 37:134-141.
[15] Iglói K, Doeller CF, Berthoz A, et al. Lateralized human hippocampal activity predicts navigation based on sequence or place memory[J]. Proc Natl Acad Sci U S A, 2010, 107(32):14467-14471.
[16] Shinohara Y, Hosoya A, Hirase H. Experience enhances gamma oscillations and interhemispheric asymmetry in the hippocampus[J]. Nat Commun, 2013, 4:1652.
[17] Tort AB, Komorowski RW, Manns JR, et al. Theta-gamma coupling increases during the learning of item-context associa-tions[J]. Proc Natl Acad Sci U S A, 2009, 106(49):20942-20947.
[18] Shirvalkar PR, Rapp PR, Shapiro ML. Bidirectional changes to hippocampal theta-gamma comodulation predict memory for recent spatial episodes[J]. Proc Natl Acad Sci US A, 2010, 107(15):7054-7059.

高脂饲养青春期大鼠电击回避反应和海马CA3区场电位的改变

The changes of electric shock avoidance response and local field potentials on hippocampal CA3 region of the adolescent rats with high fat feeding

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