白扁豆多糖对人胃癌细胞凋亡的作用及其机制

doi:10.12047/j.cjap.5663.2018.063

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (3): 268-272.doi: 10.12047/j.cjap.5663.2018.063

白扁豆多糖对人胃癌细胞凋亡的作用及其机制

张艳姿^1,2, 柯瑞君², 蒋盼若², 杨林军³, 陈佳玉^1,4

1. 温州医科大学检验学院、生命科学学院, 浙江温州 325035;
2. 绍兴文理学院医学院, 浙江绍兴 312000;
3. 台州市立医院, 浙江台州 318000;
4. 台州学院医学院, 浙江台州 318000

收稿日期:2017-12-28 修回日期:2018-02-23 出版日期:2018-05-28 发布日期:2018-09-08
通讯作者: 陈佳玉,Tel:0575-88345826;E-mail:chenyujia10@163.com E-mail:chenyujia10@163.com
基金资助:
国家自然科学基金资助项目（81402979）；浙江省科技厅资助项目（2015C33270）；浙江省卫生厅资助项目（2014KYA230）

Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

ZHANG Yan-zi^1,2, KE Rui-jun², JIANG Pan-ruo², YANG Lin-jun³, CHEN Jia-yu^1,4

1. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035;
2. Medical School, Shaoxing University, Shaoxing 312000;
3. Taizhou Municipal Hospital, Taizhou 318000;
4. Taizhou University Medical School, Taizhou 318000, China

Received:2017-12-28 Revised:2018-02-23 Online:2018-05-28 Published:2018-09-08
Supported by:
国家自然科学基金资助项目（81402979）；浙江省科技厅资助项目（2015C33270）；浙江省卫生厅资助项目（2014KYA230）

摘要/Abstract

摘要： 目的：探讨白扁豆多糖对人胃癌细胞凋亡的作用及其相关机制。方法：胃癌细胞HGC-27和SGC-7901经终浓度为16、8、4、2、1和0 μg/ml的白扁豆多糖作用24、48和72h，各设3个复孔。MTS法检测其增殖活性；分别取经4、0 μg/ml白扁豆多糖作用24h的HGC-27和SGC-7901细胞（各3个复孔），JC-1染色观察线粒体膜电位，流式细胞仪分析细胞周期和凋亡率，QPCR法探讨Bcl-2、caspase-3和Bax基因的mRNA转录水平。结果：白扁豆多糖作用后，HGC-27和SGC-7901细胞线粒体膜电位降低；细胞增殖显著受抑制（P<0.01），且效果与药物作用浓度和时间有关；细胞凋亡率分别为53.15%和38.77%，均较PBS处理组（8.07%和6.03%）明显增加（P<0.01），而细胞周期无显著变化；同时，细胞内Bcl-2基因的转录水平明显受抑制，Bax和caspase-3基因的转录明显上调（P<0.01）。结论：白扁豆多糖可通过调节Bax-Bcl-2-caspase3通路，诱导胃癌细胞HGC-27和SGC-7901凋亡。

关键词: 白扁豆多糖, 细胞调亡, 人胃癌细胞株HGC-27, 人胃癌细胞株SGC-7901

Abstract: Objective: To investigate the effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its related mechanism.Methods: Gastric cancer cell lines HGC-27 and SGC-7901 were treated with 16, 8, 4, 2, 1 and 0 μg/ml white lentil polysaccharides for 24, 48 and 72 hours respectively, each experiment 3 wells. The proliferation activities of gastric cancer cells were detected with CellTiter 96^® AQueous Non-Radioactive Cell Proliferation Assay (MTS). HGC-27 and SGC-7901 cells were treated with 0 or 4 μg/ml white lentil polysaccharides for 24 hours respectively, each experiment 3 wells, then the changes of cell mitochondrial membrane potential were observed by JC-1 staining, cell cycle and cell apoptosis rates were evaluated with flow cytometry, the levels of mRNA expression of Bcl-2, caspase-3 and Bax genes were investigated with real-time quantitative PCR detecting system (QPCR).Results: After the gastric cancer cell lines HGC-27 and SGC-7901 were treated with white lentil polysaccharides, the mitochondrial membrane potential of the cells was significantly reduced, proliferation activities of the cells were inhibited obviously (P<0.01), and the inhibition degree was related with drug concentration and incubation time. Flow cytometry analysis showed that the apoptosis rates of HGC-27 and SGC-7901 cell lines were 53.15% and 38.77% respectively, which were significantly increased compared with PBS treatment groups (8.07% and 6.03%, P<0.01). And the cell cycle of these cells was not influenced significantly. The results of QPCR showed that Bcl-2 gene transcription level of HGC-27 and SGC-7901 cell lines was significantly down-regulated, and the transcription levels of Bax and caspase-3 genes were significantly increased (P<0.01).Conclusion: The white lentil polysaccharides can induce the apoptosis of gastric cancer cells lines HGC-27 and SGC-7901, and the mechanism of their apoptosis may be mediated by the regulation of Bax-Bcl-2-caspase3 pathway.

Key words: white lentil polysaccharides, cell apoptosis, human gastric cancer cell line HGC-27, human gastric cancer cell line SGC-7901

张艳姿, 柯瑞君, 蒋盼若, 杨林军, 陈佳玉. 白扁豆多糖对人胃癌细胞凋亡的作用及其机制[J]. 中国应用生理学杂志, 2018, 34(3): 268-272.

ZHANG Yan-zi, KE Rui-jun, JIANG Pan-ruo, YANG Lin-jun, CHEN Jia-yu. Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism[J]. CJAP, 2018, 34(3): 268-272.

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白扁豆多糖对人胃癌细胞凋亡的作用及其机制

Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

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